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Detection method of concentration of isofenphos-methyl, and application

A technology of isofenphos-methyl and detection method, which is applied in the field of concentration detection, can solve the problems of time-consuming, labor-intensive, high professional knowledge requirements, complicated processing, etc.

Active Publication Date: 2018-12-21
SHENZHEN INST OF TERAHERTZ TECH & INNOVATION +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although this method has high analytical precision, the pre-processing is complicated, and many chemical reagents and equipment are used, the operation is complicated, the professional knowledge of the experimenters is high, and it is time-consuming and labor-intensive.

Method used

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  • Detection method of concentration of isofenphos-methyl, and application
  • Detection method of concentration of isofenphos-methyl, and application
  • Detection method of concentration of isofenphos-methyl, and application

Examples

Experimental program
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Effect test

Embodiment 1

[0107] The substance to be tested in the present embodiment is Shanghai green, and the method for detecting the concentration of isofosulfos-methyl of the substance to be tested in the present embodiment is as follows:

[0108] (1) Dilute the methyl isofenfos standard solution with n-hexane into a standard solution of methyl isofosphos with a concentration of 5 μg / mL, a standard solution of 20 μg / mL of methyl isofosphos, and 50 μg / mL of formazan. A standard solution of isofloxacin.

[0109] (2) Using a micropipette, pipette 80 μL of the standard solution of methyl isoflavosin with a concentration of 5 μg / mL, 80 μL of the standard solution of 20 μg / mL of methyl isoflophos with a concentration of 80 μL, and 80 μL of the concentration A standard solution of 50 μg / mL isofosporophos-methyl was placed on three quartz filters.

[0110] (3) Under the condition of nitrogen atmosphere and relative humidity less than or equal to 5%, use the terahertz time-domain spectroscopy device to d...

Embodiment 2

[0126] The detection method of the concentration of methyl isoflophos of the product to be tested in this embodiment is roughly the same as that in Example 1, the difference is that the product to be tested in this embodiment is rapeseed, and step (9) is: weighing 1 g of homogenate (with an accuracy of 0.01g) placed in a 15mL centrifuge tube, measure 8mL of acetonitrile (HPLC grade) with a pipette into the centrifuge tube, then add 3g of anhydrous magnesium sulfate, ultrasonically extract within 15 minutes, and then at 5000 rpm The filtrate was obtained by centrifugation for 5 minutes at a rotational speed of 1 / min.

[0127] Wherein, the concentration of methyl isoflophos in the to-be-tested product of the present embodiment is shown in Table 1.

Embodiment 3

[0129] The method for detecting the concentration of methyl isoflophos in the sample to be tested in this embodiment is roughly the same as that in Example 1, the difference is that the sample to be tested in this embodiment is based on the ratio that the concentration of methyl isofosphos is 25 μg / mL The Shanghai green with methyl isofenfos-methyl standard product was added, the acetonitrile added in step (9) was 9 mL, and the anhydrous sodium sulfate was 5 g. Wherein, the concentration of methyl isoflophos in the to-be-tested product of the present embodiment is shown in Table 1.

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Abstract

The invention relates to a detection method of concentration of isofenphos-methyl, and application. The detection method of the concentration of isofenphos-methyl comprises the following steps: collecting Terahertz time domain spectrum of a standard solution of each isofenphos-methyl, acquiring the terahertz absorption spectrum of the standard solution of each isofenphos-methyl, and computing areaof a feature absorption peak of the standard solution of each isofenphos-methyl; establishing a relation function of the concentration of the isofenphos-methyl and the feature absorption peak; extracting the isofenphos-methyl in the to-be-detected sample according to the dispersion solid-phase extraction method so as to obtain the purification liquid; drying the purification liquid, and then filling to the predetermined volume to obtain a detection sample, collecting the terahertz time domain spectrum of the detection sample, and acquiring the terahertz absorption spectrum of the detection sample; computing the area of the feature absorption peak of the detection sample; and computing the concentration of the isofenphos-methyl of the to-be-detected sample. The above detection method is relatively simple in operation.

Description

technical field [0001] The invention relates to the detection field of pesticides, in particular to a detection method and application of the concentration of methyl isofosphos. Background technique [0002] The hygienic testing items for vegetables generally include sulfites, nitrites, heavy metals, pathogenic bacteria and pesticide residues. Among them, pesticide residues are listed as the primary testing items, which can most directly reflect the quality of vegetables. As a highly toxic soil insecticide, it is widely used in the control of nematodes and underground pests in corn, wheat and other crops due to its wide insecticidal spectrum and long residual effect. At present, isoflophos-methyl has been listed as one of the banned pesticides in vegetable production, and it is listed as one of the pesticide varieties for quantitative monitoring of pesticide residues in vegetables, so the accuracy of the pesticide detection data of isoflophos-methyl is obvious. especially i...

Claims

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Application Information

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IPC IPC(8): G01N21/3586G01N1/34
CPCG01N1/34G01N21/3586
Inventor 王志琪李辰
Owner SHENZHEN INST OF TERAHERTZ TECH & INNOVATION
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