Preparation method of veterinary fermented ganoderma lucidum oral liquid

A Ganoderma lucidum oral liquid, veterinary fermentation technology, applied in microorganism-based methods, biochemical equipment and methods, medical preparations containing active ingredients, etc., to achieve great economic benefits, improve vaccine titers, enhance immune function and The effect of antioxidant properties

Inactive Publication Date: 2018-12-21
河南欧普生物科技有限公司
View PDF2 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, there is no report on the combination of bioengineering fermentation technology and specific traditional Chinese medicine medium screening technology to improve the fermentation of Ganoderma lucidum and produce Ganoderma lucidum triterpenes

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Preparation method of veterinary fermented ganoderma lucidum oral liquid

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] A preparation method for veterinary fermented Ganoderma lucidum oral liquid, characterized in that the following steps are carried out in sequence:

[0035] Preparation of seed solution A: Take 195g of potatoes, add 500ml of water, boil for 30 minutes and filter the residue to obtain potato liquid, add 18.8g of glucose, 9.5g of peptone, 9.5g of yeast extract, 2.0g of potassium dihydrogen phosphate, 1.0g of magnesium sulfate, and defatted silkworm chrysalis Powder 10g, Vitamin B 1 Mix 0.05g with 1000mL of water, pH6.8, sterilize at 121℃ for 30min. The method is as follows: pick 3 pieces of Ganoderma lucidum with a diameter of about 4mm and inoculate them into a conical flask with a seed medium loading capacity of 400mL / 1000mL, and culture them on a shaking table at 23°C, protected from light, and at 60rpm for 6 days to make a seed liquid of Ganoderma lucidum ,spare.

[0036] B fermentation tank enlargement: take 10% of the mixed culture of traditional Chinese medicine,...

Embodiment 2

[0040] A preparation method for veterinary fermented Ganoderma lucidum oral liquid, characterized in that the following steps are carried out in sequence:

[0041] Preparation of seed solution A: Take 200g of potatoes, add 500ml of water, boil for 30min and filter the residue to obtain potato liquid, add 18.8g of glucose, 9.5g of peptone, 9.5g of yeast extract, 2.0g of potassium dihydrogen phosphate, 1.0g of magnesium sulfate, and defatted silkworm chrysalis Powder 10g, Vitamin B 1 Mix 0.05g with 1000mL of water, pH6.8, sterilize at 121℃ for 30min. The method is as follows: Pick 3 pieces of Ganoderma lucidum with a diameter of about 4mm and inoculate them into a triangular flask with a seed medium loading capacity of 400mL / 1000mL, and culture them on a shaking table at 26°C, protected from light, and at 80rpm for 8 days to make a seed liquid of Ganoderma lucidum ,spare.

[0042] B fermentation tank enlargement: take 10% of the mixed culture of traditional Chinese medicine, v...

Embodiment 3

[0046] A preparation method for veterinary fermented Ganoderma lucidum oral liquid, characterized in that the following steps are carried out in sequence:

[0047] The formula of the seed medium described in step A is: add 200g of potatoes to 500ml of water, boil for 30min and filter the residue to obtain potato liquid, add 18.8g of glucose, 9.5g of peptone, 9.5g of yeast extract, 2.0g of potassium dihydrogen phosphate, sulfuric acid Magnesium 1.0g, defatted silkworm chrysalis powder 10g, vitamin B 1 Mix 0.05g with 1000mL of water, adjust the pH value to 7.0, and sterilize at 21°C for 30min. In addition, as a preferred shaker culture method: pick 3 Ganoderma lucidum blocks with a diameter of 4 ± 0.3mm and inoculate them into a seed medium loading bottle, and culture them on a shaker at 25°C, protected from light, and at 75rpm for 7 days to make Ganoderma lucidum Bacteria seed liquid.

[0048] The composition of the traditional Chinese medicine mixed culture in step B is: lic...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

PropertyMeasurementUnit
diameteraaaaaaaaaa
Login to view more

Abstract

A preparation method for veterinary fermented ganoderma lucidum oral liquid relates to traditional Chinese medicine fermented poultry medicine, and the following steps are sequentially performed: A, preparing seed liquid: preparing seed medium, which is sterilized for standby use; picking ganoderma lucidum strain for inoculation; the ganoderma lucidum seed liquid is obtained after the shake cultivation; B, enlarging fermenter: the ganoderma lucidum seed liquid in the step A is inoculated into the sterilized fermentation medium and the pH value is adjusted, and is sterilized; when the fermentation medium is cooled to 25 DEG C, the ganoderma lucidum seed liquid is inoculated under sterile conditions in an amount of 5-10% by volume of the fermentation broth, and cultured for 60-72 hours; finally, the mixed liquor of the fermented ganoderma lucidum is obtained; C, centrifugal solid-liquid separation: after centrifugation, obtain the supernatant and precipitate for standby use; D, the supernatant obtained after centrifugation is sterilized, detected and canned. The preparation method provided by the invention has the advantages that the bio-engineered fermentation technology is combinedwith the specific medium screening technology of the traditional Chinese medicine to prepare a fermented ganoderma lucidum oral liquid product containing a large amount of ganoderma triterpenes withthe ganoderma triterpenes content is larger than or equal to 10 mg/100 mg dry mycelium.

Description

technical field [0001] The invention relates to the technical field of biological preparations, and further relates to traditional Chinese medicine fermented poultry medicine, in particular to a preparation method of fermented Ganoderma ganoderma oral liquid for animals. Background technique [0002] Ganoderma lucidum is a fungus belonging to Basidiomycetes, Polyporaceae, and Ganoderma genus. It is a very effective higher medicinal fungus. The main active ingredient in Ganoderma lucidum is Ganoderma triterpenes. Among them, Ganoderma lucidum triterpenoids have functions of anti-inflammatory, analgesic, detoxification, liver protection, anti-HIV, anti-tumor, liver protection, inhibition of histamine release, angiotensin-converting enzyme, etc., which are the important material basis for Ganoderma lucidum to exert its medicinal effect. [0003] At present, the yield of Ganoderma lucidum mycelium and its key medicinal components has become a bottleneck hindering the applicatio...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/074A61P37/04C12N1/14C12R1/645
CPCA61K36/074A61K2236/19A61P37/04C12N1/14
Inventor 常艺海何正洁柳占稳孙凤洁
Owner 河南欧普生物科技有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap