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A method for judging the quality of blastocysts using protein chips

A protein chip, protein technology, applied in measurement devices, instruments, scientific instruments, etc., can solve the problems of insufficient blastocyst quality, lack of accurate, reliable and quantitative screening methods, and difficult objective quantification, etc., to achieve easy learning and avoidance. Human error, avoid poor repeatability

Inactive Publication Date: 2019-10-11
NORTHWEST WOMEN & CHILDREN HOSPITAL
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  • Claims
  • Application Information

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Problems solved by technology

Therefore, there are obvious deficiencies in the screening of blastocyst quality by morphological scoring, which is difficult to objectively quantify, and there is no accurate, reliable, and quantitative screening method

Method used

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  • A method for judging the quality of blastocysts using protein chips
  • A method for judging the quality of blastocysts using protein chips
  • A method for judging the quality of blastocysts using protein chips

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Embodiment Construction

[0050] Such as figure 1 As shown, taking the detection method of a blastocyst culture medium as an example to illustrate, a method for judging the quality of blastocysts using a protein chip of the present invention comprises the following steps:

[0051] Step 1. Place the embryos that require quality judgment in an embryo culture dish for embryo culture. When the embryo develops to the blastocyst stage, that is, on the 5th to 6th day after fertilization, transfer the blastocyst to the culture droplet of another embryo culture dish ;

[0052] Step 2, suck the culture liquid in the embryo culture dish after the blastocysts are transferred in step 1 into the sample tube, and after adding the protease inhibitor in the sample tube, mix the two evenly to make the sample to be tested; The volume ratio of the culture drop added in the sample tube to the protease inhibitor is 6:1 to 9:1, and the protease inhibitor is 10× protease inhibitor;

[0053] Step 3, add the sample to be test...

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Abstract

The invention discloses a method of judging quality of a blastocyst by using a protein chip. The method comprises the following steps: I, when an embryo is developed to the blastocyst stage, transferring the blastocyst; II, dropping and absorbing a culture liquid to a sample tube and adding a protease inhibitor into the sample tube to prepare a to-be-tested sample; III, adding the to-be-tested sample into a 384 porous plate, adding a sample application liquid into each hole to prepare a to-be-sample applied sample; IV, dropping the to-be-sample applied sample in each hole to the chip, puttingthe chip in a wet box and drying the chip in an oven; V, incubating a protein antibody for the dried chip to prepare the protein chip; and VI, carrying out fluorescence detection on each protein chipand comparing the contents of six proteins of each blastocyst with standard contents of the six proteins. The method can evaluate the development condition of the blastocyst noninvasively, objectivelyand quantitatively, can be used for screening blastocysts which are high in quality and have planting potential and helps to improve the accuracy of screening the quality of the blastocyst.

Description

technical field [0001] The invention belongs to the technical field of blastocyst development evaluation technology, and in particular relates to a method for judging blastocyst quality by using a protein chip. Background technique [0002] According to the statistics of the World Health Organization, the incidence rate of infertility among the population reaches 10% to 15%, and it is on the rise. At present, the most important and efficient means of treating infertility is in vitro fertilization-embryo transfer (In vitro fertilization-Embryo transfer, IVF-ET) technology, that is, test-tube baby. This technology is a series of artificial assisted pregnancy technologies that fertilize human sperm and eggs in vitro, culture embryos in vitro, and transfer embryos back to the uterus. [0003] In IVF-ET technology, it is often necessary to screen multiple embryos of each patient, and select the embryo with the best potential for implantation and development for transplantation. ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/68G01N33/533G01N33/58
Inventor 施文浩
Owner NORTHWEST WOMEN & CHILDREN HOSPITAL
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