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Tripeptides with ace inhibitory activity and applications thereof

A technology for inhibiting activity and drugs, applied in tripeptides with ACE inhibitory activity and its application fields, can solve problems such as difficult and complex biologically active peptides, and achieve easy absorption, overcome digestion in the gastrointestinal tract, good potential and application prospects Effect

Active Publication Date: 2021-06-25
BOHAI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, it is complicated and difficult to separate and purify high-purity and high-activity bioactive peptides from protein hydrolysates

Method used

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  • Tripeptides with ace inhibitory activity and applications thereof
  • Tripeptides with ace inhibitory activity and applications thereof
  • Tripeptides with ace inhibitory activity and applications thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Example 1 Virtual screening and activity verification of large yellow croaker-conactin ACE inhibitory peptide

[0027] Based on the online program ExPASy PeptideCutter, three typical gastrointestinal digestive proteases, pepsin (EC 3.4.23.1), trypsin (EC 3.4.21.4) and chymotrypsin (EC 3.4.21.1) were used to treat large yellow croaker muscle Kinetin (Accession of NCBI: KKF11904.1) was subjected to simulated enzymatic hydrolysis. ToxinPred, peptide property calculator, PeptideRanker and admetSAR were used to predict toxicity, water solubility, biological activity and ADME properties of unreported tripeptide sequences. A tripeptide with non-toxicity, good solubility, activity score higher than 0.5 and good intestinal permeability was screened, and further screened by molecular docking with ACE (PDB ID: 1O86) as the protein target. Combined with the key amino acids of CDOCKER score and function, the tripeptide HGR with theoretical potential ACE inhibitory activity was scre...

Embodiment 2

[0028] Example 2 Virtual screening and activity verification of ACE inhibitory peptide of large yellow croaker moesin

[0029] Based on ExPASy PeptideCutter, an online virtual enzyme digestion tool, pepsin (EC3.4.23.1) and trypsin (EC 3.4.21.4) were used to perform virtual enzyme digestion on the moesin of large yellow croaker (Accession of NCBI: KKF16686), and passed The online tools ToxinPred, peptide property calculator, PeptideRanker and admetSAR predict the toxicity, water solubility, biological activity and ADME properties of unreported tripeptide sequences. A tripeptide with non-toxicity, good solubility, activity score higher than 0.5 and good intestinal permeability was screened, and further screened by molecular docking with ACE (PDB ID: 1O86) as the protein target. Combined with the key amino acids of CDOCKER score and function, the tripeptides WAK and CMK with theoretical potential ACE inhibitory activity were screened. The results showed that WAK could bind to th...

Embodiment 3

[0030] Example 3 Virtual screening and activity verification of large yellow croaker collagen ACE inhibitory peptide

[0031] Based on the ExPASy PeptideCutter online virtual digestion tool, three typical gastrointestinal digestive proteases, pepsin (EC 3.4.23.1), trypsin (EC 3.4.21.4) and chymotrypsin (EC 3.4.21.1) were used to Yellow croaker collagen (Accession of NCBI: KKF14511) was subjected to virtual digestion, and the toxicity, water solubility, biological activity and ADME properties of unreported tripeptide sequences were predicted by online tools ToxinPred, peptide property calculator, PeptideRanker and admetSAR. A tripeptide with non-toxicity, good solubility, activity score higher than 0.5 and good intestinal permeability was screened, and further screened by molecular docking with ACE (PDB ID: 1O86) as the protein target. Combined with the key amino acids of CDOCKER score and function, the tripeptides AMR and LEW with theoretical potential ACE inhibitory activity ...

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Abstract

The invention discloses a tripeptide with ACE inhibitory activity and application thereof. The ACE inhibitory tripeptides have amino acid sequences of HGR, WAK, CMK, AMR, LEW, and FPK, respectively, and molecular weights of 368.40, 403.49, 380.52, 376.48, 446.51, and 390.49 Da. The ACE inhibitory peptide provided by the present invention has a significant inhibitory effect on ACE, and has the characteristics of being safe, non-toxic and side effects, and easy to absorb. very important.

Description

technical field [0001] The invention belongs to the field of biotechnology, and specifically relates to tripeptides with ACE inhibitory activity and applications thereof. Background technique [0002] Angiotensin-converting enzyme (ACE) is a key enzyme that regulates blood pressure and plays a key role in the renin-angiotensin-aldosterone system and kallikrein-kinin system. It converts the inactive form of angiotensin I (ANG I; Asp-Arg-Val-Tyr-Ile-His-Pro-Phe-His-Leu) into the vasoconstrictor active angiotensin II (ANGII; Asp -Arg-Val-Tyr-Ile-His-Pro-Phe) and lead to the degradation of bradykinin. Currently, synthetic ACE inhibitors such as captopril, enalapril and lisinopril have been used clinically to treat hypertension. However, synthetic ACE inhibitors pose potential health hazards. Therefore, ACE inhibitors of natural origin have recently received increasing attention. [0003] Bioactive peptides are fragments that are cleaved from protein sequences and have physio...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K5/097C07K5/083C07K5/08C07K5/087C07K5/093A23L33/18
CPCA23V2002/00A23L33/18C07K5/0802C07K5/0808C07K5/0812C07K5/0821A23V2200/30A23V2200/32A23V2250/55
Inventor 于志鹏武思佳赵文竹邱卓楠
Owner BOHAI UNIV
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