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Color retaining stationary liquid used for anatomy teaching specimens, and preparation method thereof

A color fixation and specimen technology, applied in the field of specimen fixative, can solve the problems of irritating, toxic, flammable and explosive, affecting the fidelity of specimens, fading specimens, etc., achieve remarkable environmental protection characteristics, improve production quality and production efficiency , long color retention effect

Active Publication Date: 2018-12-04
山东聚众数字医学科技开发有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

That is to say: Most of these traditional fixative components have use defects such as irritation, toxicity, volatility, flammability and explosiveness.
At the same time, during the soaking and use of the existing fixatives for animal specimens, there are various degrees of fading and dimming of the specimens.
Namely: when the animal soaked specimen is soaked in the fixative for a long time, the appearance of the animal tissue will fade to varying degrees due to bacterial invasion, colony reproduction, cell oxidation and other reasons, which will affect the fidelity of the specimen

Method used

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Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0026] A method for preparing color-preserving fixative for anatomical teaching specimens, including the following steps:

[0027] (1) Preparation of citrus fruit peel extract

[0028] Take the peels of citrus fruits, wash them and dry them, and place them in a crusher to crush them into a slurry. Then, add 0.3-0.7% cellulase and 0.2-0.4% fruit to the slurry. After stirring the peptase evenly, carry out enzymatic hydrolysis treatment at 40-50℃ for 1.5-2.5h. During enzymatic hydrolysis treatment, ultrasonic treatment is carried out for 20-30S every 30 minutes. After that, under constant stirring, Add NaOH solution to the resulting enzymatic hydrolysis product until the pH of the resulting mixture is 10-11, and then vacuum filter, take the filtrate, and under constant stirring, assist in ultrasonic vibration treatment with a frequency of 55-65KHz, and add to the filtrate Add acid-base regulator acetic acid solution or phosphoric acid solution, adjust the pH of the filtrate to 2-3, r...

Embodiment 1

[0037] A color-preserving fixative for anatomical teaching specimens, composed of the following parts by weight: 2.5 parts of sodium lauryl sulfate, 12 parts of urea, 0.5 parts of potassium sorbate, 0.3 parts of citric acid, 1,2-propanediol 20 parts, 0.6 parts of sodium tripolyphosphate, 0.5 parts of ascorbic acid, 0.2 parts of L-cysteine, 1 part of citrus fruit peel extract, 1.2 parts of tea residue extract, and 65 parts of distilled water.

[0038] A method for preparing color-preserving fixative for anatomical teaching specimens, including the following steps:

[0039] (1) Preparation of citrus fruit peel extract

[0040] Take the peels of citrus fruits, wash them and dry them, place them in a crusher and break them into a slurry. Then, add 0.6% by mass of cellulase and 0.3% by mass of pectinase to the slurry, and stir After homogenization, carry out enzymatic hydrolysis treatment at 45℃ for 2h. During enzymatic hydrolysis treatment, ultrasonic treatment is carried out for 25S ev...

Embodiment 2

[0048] A color-preserving fixative for anatomical teaching specimens, composed of the following parts by weight: 2 parts of sodium lauryl sulfate, 15 parts of urea, 0.3 parts of potassium sorbate, 0.2 parts of citric acid, 1,2-propanediol 18 parts, 0.7 parts of sodium tripolyphosphate, 0.4 parts of ascorbic acid, 0.1 parts of L-cysteine, 1.3 parts of citrus fruit peel extract, 1 part of tea residue extract, and 60 parts of distilled water.

[0049] A method for preparing color-preserving fixative for anatomical teaching specimens, including the following steps:

[0050] (1) Preparation of citrus fruit peel extract

[0051] Take the peels of citrus fruits, wash them and dry them, place them in a crusher and break them into a slurry. Then, add 0.7% by mass of cellulase and 0.2% by mass of pectinase to the slurry, and stir After homogenization, carry out enzymatic hydrolysis at 40°C for 2.5 hours. During enzymatic hydrolysis, ultrasonic treatment is carried out for 30 seconds every 30 ...

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Abstract

The invention discloses a color retaining stationary liquid used for anatomy teaching specimens, and a preparation method thereof. The color retaining stationary liquid used for anatomy teaching specimens is composed of, by weight, 2 to 3 parts of sodium dodecyl sulfate, 10 to 15 parts of urea, 0.3 to 0.7 part of potassium sorbate, 0.2 to 0.5 part of citric acid, 18 to 23 parts of 1, 2-propylene glycol, 0.5 to 0.7 part of sodium tripolyphosphate, 0.4 to 0.6 part of ascorbic acid, 0.1 to 0.3 part of L-cysteine, 0.8 to 1.3 parts of orange fruit pericarp extract product, 1 to 1.5 parts of tea residue extract product, and 60 to 67 parts of distilled water. The color retaining stationary liquid can be used for rapid penetration and filling of animal specimen cells, possesses excellent bacteriainhibiting and killing effect, mildew resistance, color retaining effect, and oxidation resistance, is capable of improving animal solution preserved specimen stationary quality, effect, and color, and prolonging preservation time, is safe, is nontoxic, and is friendly to the environment.

Description

Technical field [0001] The invention belongs to the technical field of specimen fixatives, in particular to a color-preserving fixative for anatomy teaching specimens and a preparation method thereof. Background technique [0002] There are many types and varieties of animal teaching specimens, including immersion specimens, plasticized specimens, peeled specimens, transparent specimens, bone specimens, dried specimens, pathological tissue specimens, etc. They play an important role in scientific research, teaching, and popular science. effect. Among them, immersion specimens have the longest history of application and a wide range because of their simple production method and easy operation. The preparation steps of the immersed specimen include peeling, removing fat, residual meat, connective tissue, stained blood, etc., followed by washing, degreasing, tanning, neutralization, and washing, and then fixing in a fixative. [0003] The fixative components traditionally used for f...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N1/28
CPCG01N1/28
Inventor 郑彬龙王广欣郭均兵张学森
Owner 山东聚众数字医学科技开发有限公司
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