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Preparation method of poultry vaccine dilution liquid

A technology of diluent and vaccine is applied in the field of preparation of vaccine diluent for poultry, which can solve the problems of sterility and inability to achieve the effect of ensuring the safety of use.

Inactive Publication Date: 2018-11-30
派生特(福州)生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, although the vaccines on the market use the transfer factor diluent to dilute the vaccine, some poultry vaccines cannot be completely sterile after being diluted with the transfer factor diluent.

Method used

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  • Preparation method of poultry vaccine dilution liquid
  • Preparation method of poultry vaccine dilution liquid

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Effect test

preparation example Construction

[0022] The invention provides a method for preparing poultry vaccine diluent, comprising the following steps:

[0023] 1) mixing chicken bursa, chicken intestines and water and then homogenizing to obtain a homogenate;

[0024] 2) The homogenate in step 1) was repeatedly frozen and thawed 6 to 8 times to obtain a freeze-thaw solution;

[0025] 3) Stirring and centrifuging the mixed solution obtained by mixing the freeze-thaw solution of the step 2) with acetic acid in sequence, and mixing the obtained centrifuged liquid with β-propiolactone for inactivation to obtain an inactivation solution;

[0026] 4) hydrolyzing the inactivation solution in step 3) at 35-42°C for 1-3 hours to obtain a hydrolyzate;

[0027] 5) performing microfiltration on the hydrolyzate in step 4), adjusting the pH value of the obtained microfiltrate to 6.5 to 7.5 to obtain a conditioning solution;

[0028] 6) Ultrafiltration is performed on the conditioning solution in step 5), and the resulting ultraf...

Embodiment 1

[0047] (1) Clean the chicken bursa and chicken intestines, remove the skin, remove fat and tendons, and wash them with water for injection for later use. The chicken bursa and chicken intestines are all from fresh healthy animals;

[0048] (2) Put 5Kg of processed chicken bursa and 1Kg of chicken intestines into a meat grinder and grind them, then add water for injection to the obtained grounds at a volume ratio of 1:1, and place them in a grinder at a speed of 15000rpm Homogenize for 20 minutes to obtain a homogenate;

[0049] (3) The homogenate was repeatedly frozen and thawed 6 times (the freezing temperature during freezing and thawing was -22°C, and the time was 48h, and the temperature during dissolution was 30°C, and the time was 1.5h), to obtain a freeze-thaw liquid;

[0050](4) After adding acetic acid with a final concentration of 1%, the freeze-thaw solution was stirred at 30 rpm for 14 hours at 10°C, then placed at 4°C, centrifuged at 5000rpm for 20 minutes, and th...

Embodiment 2

[0054] (1) Clean the chicken bursa and chicken intestines, remove the skin, remove fat and tendons, and wash them with water for injection for later use. The chicken bursa and chicken intestines are all from fresh healthy animals;

[0055] (2) Put 5Kg of processed chicken bursa and 1Kg of chicken intestines into a meat grinder and grind them, then add water for injection to the obtained grounds at a volume ratio of 1:2, and place them in a masher at a speed of 16500rpm Homogenize for 15 minutes to obtain a homogenate;

[0056] (3) The homogenate was repeatedly frozen and thawed 7 times (the freezing temperature during freezing and thawing was -20°C and the time was 52h, and the temperature during dissolution was 25°C and the time was 1.5h) to obtain a freeze-thaw solution;

[0057] (4) Add acetic acid with a final concentration of 2% to the freeze-thaw solution, stir at 50 rpm for 10 hours at 10°C, then place at 0°C, centrifuge at 10,000 rpm for 10 minutes, take the supernatan...

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Abstract

The invention provides a preparation method of a poultry vaccine dilution liquid, and belongs to the technical field of biomedicine. The preparation method comprises: mixing chicken bursa, chicken intestine and water, homogenizing, carrying out repeated freezing-thawing on the obtained homogenized liquid, mixing the obtained freezing-thawing liquid and acetic acid, sequentially carrying out stirring and centrifugation, mixing the obtained centrifugation supernatant and beta-propiolactone, inactivating, hydrolyzing the obtained inactivated liquid for 1-3 h at a temperature of 35-42 DEG C, carrying out micro-filtration on the obtained hydrolyzate, adjusting the pH value of the obtained micro-filtrate, carrying out ultra-filtration on the obtained adjusted liquid, and sterilizing the obtainedultra-filtrate to obtain the poultry vaccine dilution liquid. According to the present invention, the poultry vaccine dilution liquid prepared through the preparation method can completely kill the non-specific pathogenic bacteria of poultry vaccine, and can ensure the safe use of the vaccine.

Description

technical field [0001] The invention relates to the technical field of biomedicine, in particular to a preparation method of poultry vaccine diluent. Background technique [0002] Vaccines refer to biological products made of various pathogenic microorganisms for vaccination. The purpose of immunization is to induce the body to produce a strong immune response to the inoculation antigen, so as to protect the body from the invasion of corresponding pathogens. The use of vaccines is very popular today, and both humans and livestock need to be vaccinated to prevent certain diseases. At present, although the vaccines on the market use the transfer factor diluent to dilute the vaccine, some poultry vaccines cannot be completely sterile after being diluted with the transfer factor diluent. Contents of the invention [0003] The object of the present invention is to provide a method for preparing poultry vaccine diluent. The diluent prepared by the preparation method provided by...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K47/42A61K47/46
CPCA61K47/42A61K47/46
Inventor 刘小龙叶盛聪邱灵珊陈胜勇刘楚楚徐磊刘友霖
Owner 派生特(福州)生物科技有限公司
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