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A kind of norditerpene compound and its preparation method and application

A compound, the technology of norditerpene, applied in the fields of medicinal chemistry and microbial medicine, can solve the problems of no literature reports

Active Publication Date: 2021-12-07
INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] According to literature research, the two norditerpene compounds involved in the present invention are new compounds, and there is no literature report before

Method used

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  • A kind of norditerpene compound and its preparation method and application
  • A kind of norditerpene compound and its preparation method and application
  • A kind of norditerpene compound and its preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Embodiment 1. Fermentative production and separation and purification of compounds 1 and 2 shown in formula I:

[0024] The marine fungus Aspergillus wentii (Aspergillus wentii) (size 2.5 cm × 2.5 cm) was inoculated in 300 mL PDB liquid medium grown in plate culture medium, and fermented on a shaker (200 rpm) at 28°C for 7 days. The obtained bacterial liquid was inserted into a 30L PDB liquid medium, fermented in a 50L fermenter for 1 day, then inserted into a 300L PDB liquid medium as a seed liquid and fermented in a 500L fermenter for 7 days, and the fermentation product was repeatedly soaked and extracted with ethyl acetate, combined The extract is concentrated to obtain a fermented crude extract

[0025] The formula of the PDB liquid medium is: 200 grams of potato powder, 20 grams of glucose, 5 grams of peptone, 3 grams of yeast extract powder and 35 grams of sea salt per liter of water.

[0026] The crude extract was subjected to reduced-pressure silica gel column...

Embodiment 2

[0034] Example 2. Activity of inhibitors against aquatic pathogens.

[0035] The antibacterial activity of compounds 1 and 2 represented by formula I was detected by the minimum inhibitory concentration method. The following three aquatic pathogenic strains were selected: Edwardsiella tarda, Vibrio harveyi or Vibrio parahaemolyticus for antibacterial activity test.

[0036] 1) Antibacterial activity test (MIC method):

[0037] Minimum Inhibitory Concentration (MIC), the lowest concentration of a drug that can inhibit bacterial growth in vitro. In a 96 microwell plate, by adding different concentrations of drugs into the bacterial suspension of the bacteria to be tested, observe after incubation, if the indicator bacteria grow in a certain well, it means that the drug concentration in the well cannot inhibit the growth of the bacteria, The liquid in the hole is turbid, and the light transmittance drops obviously. On the contrary, the liquid in the hole is clear, and the decr...

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Abstract

The invention relates to the technical fields of medicinal chemistry and microbial medicine, in particular to a method for obtaining norditerpenoids from the fermentation product of the marine fungus Aspergillus wentii and its separation and purification method and the activity of inhibiting aquatic disease bacteria application. Described norditerpene compound is compound 1 and compound 2 shown in formula I, and compound 1 and compound 2 are a pair of isomers, and its molecular formula is C 19 h 26 o 3 The antibacterial activity test shows that compound 1 and compound 2 have significant inhibitory activity to aquatic disease bacteria Edwardsiella tarda, Vibrio harveyi and Vibrio parahaemolyticus, and its minimum inhibitory concentration (MIC) Both are 8.0μg / mL, and it is expected to develop into a new type of aquatic disease control drug.

Description

technical field [0001] The invention relates to the technical fields of medicinal chemistry and microbial medicine, in particular to a method for obtaining norditerpene compounds from the fermentation product of the marine fungus Aspergillus wentii and its preparation method for separation and purification, and for inhibiting the activity of aquatic disease bacteria application. Background technique [0002] Marine microbial secondary metabolites have rich structural diversity and significant biological activity, and are an important source of drug lead compounds. Norditerpene compounds with a 20-Nor-isopimarane skeleton structure are a class of biologically active compounds mainly produced by fungi. [0003] According to literature research, the two norditerpene compounds involved in the present invention are new compounds, which have not been reported in the literature before. Contents of the invention [0004] The object of the present invention is to provide a nordit...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07C49/743C12P7/26A01P1/00C12R1/66
CPCC07B2200/07C07C49/743C12P7/26C07C2603/26
Inventor 王斌贵李晓栋李鑫李晓明
Owner INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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