Method, enrichment probe and detection primer for rapidly detecting ALK fusion gene

Abstract

The invention provides a method, an enrichment probe and a detection primer for rapidly detecting an ALK fusion gene and belongs to the field of gene detection. The method comprises the following steps: firstly setting the enrichment probe of SEQ ID: 1-28 and capturing specifically-matched DNA, then setting any pair of detection primers of SEQ ID: 29-50, and detecting whether the ALK fusion gene exists or not through a PCR method. The method is used for detecting the ALK fusion gene based on the enrichment probe and the detection primer, has the advantages of rapid and accurate detection, lowrequirements on samples, high sensitivity and high specificity, and is capable of greatly improving the fine treatment level of lung cancer.

Description

technical field [0001] The invention belongs to the field of gene detection, and in particular relates to a method for rapidly detecting ALK fusion gene, enrichment probes and detection primers. Background technique [0002] Fusion gene refers to the process in which all or part of the sequences of two genes are fused into a new gene, which is usually carcinogenic, especially important for fusion genes such as ALK in lung cancer. The traditional fusion gene detection method is fluorescence in situ hybridization method, which must use tissue samples for detection, and this method has disadvantages such as low detection throughput, high requirements for personnel for manual interpretation, and high requirements for sample quality, which limit its application. With the development of next-generation sequencing technology, a new method for detecting fusion genes has been derived based on the method of next-generation sequencing, which has high sensitivity, high throughput, objec...

AI Technical Summary

Problems solved by technology

The traditional fusion gene detection method is fluorescence in situ hybridization method, which must use tissue samples for detection, and this method has disadvantages such as low detection throughput, high requirements for personnel and high sample quality for manual interpretation, which limit its application

With the development of next-generation sequencing technology, a new method for detecting fusion genes has been derived based on the method of next-generation sequencing, which has high sensitivity, high throughput, objective and accurate judgment of results, and can detect traditional tissue samples and detect circulating tumor DNA at the same time Samples and other advantages, but based on the current level of sequencing instrument technology, there are disadvantages such as long detection cycle and high cost

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