Double-layer running gel dyeing device, automatic running gel drying device and use method thereof

Abstract

The invention provides a double-layer running gel dyeing device, an automatic running gel drying device and a use method thereof. The double-layer running gel dyeing device comprises a first vessel and a second vessel, wherein the first vessel is stored in the second vessel; a gap is formed between the outer wall of the first vessel and the inner wall of the second vessel; a plurality of through holes are formed in the side edge of the bottom part of the first vessel; an upper cover covers the upper end of the second vessel. The invention also provides the use method of the double-layer running gel dyeing device. The method is simple and convenient to operate, high in adaptability, high in agent utilization rate, free from running gel pollution, high in safety, and high in sealing performance; the following dyeing steps of the running gel technology are conveniently carried out, and the effect is obviously superior to that of a pressing tilting method, a shaking liquid draining method,a flat supporting pouring method, a siphon method and other methods in current practice. The invention also provides the automatic running gel drying device. The automatic running gel drying device is high in automation degree; the operation process can be further simplified; and the device has important reality value, scientific significance and high industrialization prospect.

Description

technical field [0001] The invention belongs to the field of molecular biology, in particular to a double-layer electrophoresis gel dyeing device, an automatic electrophoresis gel dyeing device and a use method. Background technique [0002] The electrophoresis gel staining technology is mainly aimed at the staining of protein gels and DNA gels. The commonly used electrophoresis gel staining methods mainly include dye staining (such as test staining), silver staining, negative staining, and fluorescent staining. [0003] At present, the sensitivity of various electrophoretic gel staining techniques is relatively high. Various interferences in the staining process may easily cause impurities in the electrophoretic gel and affect the experimental results. In addition, the dyeing process is cumbersome and the operation is complicated. Not only is it easy to contaminate the electrophoretic gel and it is easy to cause waste of reagent phenomenon; and direct contact with reagents ...

AI Technical Summary

Problems solved by technology

[0005] At the same time, various staining methods require the use of containers containing electrophoretic gel, and often require multiple operations such as adding liquid and draining liquid. In practice, some operators use containers with shallow edges (such as iron plates, ceramic plates, etc.) Packing, remove the water in the container by shaking the container, this method will cause a large amount of solution to remain in the container, affecting the dyeing effect

There are also experimenters who hold the edge of the electrophoretic gel with their hands, and then pour out the water in the device. This method will leave fingerprints on the gel, affect imaging, and easily cause the gel to break

In the research process, the inventor tried to use the siphon method and the method of pouring the staining solution after lifting the electrophoresis gel with a flat plate, but found that these two methods were slow and inconvenient to operate, and the siphon tube was easy to pollute the electrophoresis gel, The dyeing solution cannot be completely absorbed, and the electrophoretic gel may be easily broken if the pallet is not properly operated.

Another major inconvenience of electrophoresis gel staining is that the experimenters need to observe the electrophoresis results at any time, and add or discharge the staining solution regularly and quantitatively, which greatly consumes manpower

[0006] At present, the dyeing devices used in the dyeing process have certain problems, such as cumbersome operation, waste of reagents, contamination of samples, etc.

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