Kit for detecting excrement exfoliative cell DNA methylation state to analyze colorectal cancer

Abstract

The invention discloses a kit for detecting an excrement exfoliative cell DNA methylation state to analyze colorectal cancer and specifically provides a kit for detecting colorectal cancer related PDX1 gene methylation from colorectal cancer excrement exfoliative cells and application thereof. According to the kit disclosed by the invention, a specific methylation primer is designed to detect a nucleotide sequence of PDX1 gene methylation to achieve screening and auxiliary diagnosis of colorectal cancer. A fluorescent quantitative PCR method is utilized to analyze a methylation level of a PDX1gene marker, and a positive result or a negative result of a sample can be judged according to a reported CT value. In clinical researches, absolute majority of colorectal cancer and most precancerous adenoma can be detected; 90% of early colorectal cancer can be detected, 64% of precancerous adenoma (larger than or equal to 1 centimeter) can be detected, and specificity is 100%; furthermore, wound is completely avoided, only 4.5 grams of excrement is needed, and acceptability by crowds without symptoms is high.

Description

technical field [0001] The invention belongs to the field of molecular biology, and relates to a kit for screening colorectal cancer by a methylation-specific PCR method, which is suitable for detecting whether there is colorectal cancer mutation DNA in exfoliated cells of feces. Background technique [0002] Colorectal cancer is one of the most common malignant tumors in humans, and its incidence rate ranks third among malignant tumors in the world. According to the statistics of the World Health Organization, the annual incidence of colorectal cancer in the world is 1.2 million, and the annual mortality rate is about 600,000. The incidence of colorectal cancer is stabilizing in developed countries. In developing countries headed by China, with the changes in people's lifestyle and diet structure, the incidence of colorectal cancer is on the rise in a straight line. Statistics show that the annual growth rate is about 4.2%, and it has become the third place among women and...

AI Technical Summary

Problems solved by technology

Although CS has the advantages of good curative effect and high accuracy, it also has certain limitations: (1) CS is an invasive examination method with a higher risk of complications, which may cause colonic perforation (0.3%) or even death (1 / 5000); (2) It is time-consuming and requires adequate bowel preparation and sedation before the examination; (3) It is more expensive, and it will cost more if tissue biopsy and resection of the found polyps are required; (4) Colonic The missed diagnosis rate of colorectal cancer by endoscopy is 5%

However, as a routine screening method, CTC has obvious deficiencies: In order to obtain a satisfactory image, 2-4 rads of X-rays still need to be released to the abdomen of the patient during the CTC examination, which increases the risk of cancer; Air or carbon dioxide is injected into the intestinal lumen to expand the intestinal tract, and the patient will experience some discomfort during the examination; in addition, the high examination fee will bring a heavier economic burden to both the individual and the society

However, at present, there are few studies on the correlation between colorectal cancer and PDX1 gene methylation, and there is no relevant research report on the detection kit of PDX1 gene methylation to detect or screen colorectal cancer

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