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Method for extracting protein from sludge by low-intensity ultrasonic enhanced enzymolysis

An ultrasonic and low-intensity technology, which is applied in the field of low-intensity ultrasonic-enhanced enzymatic sludge protein extraction, can solve the problems of low extraction efficiency of sludge protein, achieve good protein extraction effect, improve protein extraction effect, and improve efficiency

Pending Publication Date: 2018-11-06
ZHENGZHOU UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0007] The purpose of the present invention is to provide a low-intensity ultrasonic-enhanced method for extracting protein from sludge by enzymatic hydrolysis in order to solve the problem that the efficiency of extracting sludge protein by enzymatic hydrolysis is too low

Method used

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  • Method for extracting protein from sludge by low-intensity ultrasonic enhanced enzymolysis

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Embodiment 1

[0036] In this example, low-intensity ultrasonic waves combined with enzymatic methods are used to extract sludge protein: take sludge with a water content of 96%, put it in a 300mL beaker, add NaOH solution to adjust the pH value of the sludge to 11, and then add 2000U / g of alkaline Protease, stirred evenly and placed in the center of the ultrasonic cleaning tank; irradiated ultrasonically for 40 minutes at a sound energy density of 0.08W / mL; then placed the ultrasonically treated sludge in a constant temperature oscillator at 65°C for 2 hours; After the end, place the sludge in a boiling water bath for inactivation for 10 minutes, and then perform centrifugal filtration on the sludge after inactivation, and test the protein content in the sludge filtrate.

Embodiment 2

[0038] In this example, low-intensity ultrasonic waves combined with enzymatic methods are used to extract sludge protein: take sludge with a moisture content of 95% and place it in a 300mL beaker, add NaOH solution to adjust the pH value of the sludge to 10, and then add 3000U / g alkaline Protease, stirred evenly and placed in the center of the ultrasonic cleaning tank; irradiated ultrasonically for 25 minutes at a sound energy density of 0.15W / mL; then placed the ultrasonically treated sludge in a constant temperature oscillator at 55°C for 3.5 hours; After the reaction, the sludge was placed in a boiling water bath for inactivation for 15 minutes. After inactivation, the sludge was subjected to centrifugal filtration treatment, and the protein content in the sludge filtrate was tested.

Embodiment 3

[0040] In this example, low-intensity ultrasonic waves combined with enzymatic methods are used to extract sludge protein: take sludge with a water content of 94%, put it in a 300mL beaker, add NaOH solution to adjust the pH value of the sludge to 9, and then add 6000U / g of alkaline Protease, stirred evenly and placed in the center of the ultrasonic cleaning tank; irradiated ultrasonically for 15 minutes at a sound energy density of 0.175W / mL; then placed the ultrasonically treated sludge in a constant temperature oscillator at 45°C for 5 hours; After the end, place the sludge in a boiling water bath to inactivate for 20 minutes. After inactivation, the sludge is subjected to centrifugal filtration treatment, and the protein content in the sludge filtrate is tested.

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Abstract

The invention belongs to the field of urban sludge resource treatment, and discloses a method for extracting protein from sludge by low-intensity ultrasonic enhanced enzymolysis. The method comprisesthe followings steps: firstly, adding an enzyme preparation to the sludge, then placing the sludge with the enzyme preparation in a low-intensity ultrasonic system to enhance activity of enzyme, and placing the enhanced enzyme and sludge in a hydrolysis system for enzymolysis reaction. Activity of the enzyme preparation is enhanced by the low-intensity ultrasonic system, so that the reaction process of enzyme hydrolysis on sludge is further promoted, reaction time of sludge enzymolysis is shortened, the extraction rate of protein from the sludge is increased, energy consumption during sludge treatment by ultrasonic combined with enzyme is reduced, and the method is an economical and efficient method for extracting protein from the sludge.

Description

technical field [0001] The invention belongs to the field of resource treatment of urban sludge, and specifically relates to a method for extracting protein from sludge with low-intensity ultrasonic intensification by enzymolysis. Background technique [0002] With the development of urbanization and the continuous improvement of living and production capacity, the amount of sewage treatment continues to increase, and the corresponding residual sludge, a by-product of sewage treatment, also increases significantly. From a microscopic point of view, sludge has complex components and is difficult to degrade; from a macroscopic point of view, sludge particles are small, have high moisture content, and occupy a large area. The data shows that based on a moisture content of 80%, the annual sludge production in 2017 reached more than 50 million tons. The protein resources contained in the urban surplus sludge are rich (about 30-60%), and the resource utilization of the sludge can...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/06
CPCC12P21/06
Inventor 闫怡新高健磊丁静雨高镜清岳秋彩李超
Owner ZHENGZHOU UNIV
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