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Method to quickly detect alpha-fetoprotein

A fast technology for alpha-fetoprotein, applied in the direction of measuring devices, instruments, and material analysis through electromagnetic means, can solve the problems of long time-consuming and cumbersome, and achieve fast, easy-to-operate, and low-cost effects

Inactive Publication Date: 2018-10-26
EAST CHINA UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The detection method of alpha-fetoprotein in the prior art needs to be prepared through a relatively cumbersome solution, and the combination and reaction time exceeds 20 hours (such as CN103558396A), which takes a long time

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  • Method to quickly detect alpha-fetoprotein
  • Method to quickly detect alpha-fetoprotein
  • Method to quickly detect alpha-fetoprotein

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] The carboxylated single-walled carbon nanotubes were dispersed into a 1% poloxamer solution to form a dispersion with a concentration of 3 mg / mL. Mix 5 mL of single-walled carbon nanotube dispersion with 1 μL of alpha-fetoprotein antibody to form a single-walled carbon nanotube-alpha-fetoprotein complex. Add the single-walled carbon nanotube-alpha-fetoprotein complex dropwise into the printer cartridge and wait for printing. At the same time, the detection area of ​​the paper-based sensor is designed by drawing software. The outline of the paper chip is printed first, followed by the hydrophobic areas. Finally, the printed paper-based sensor was placed in a 37°C oven for about 24 hours to obtain a paper-based sensor ( figure 1 ). The paper-based sensor obtained by this method can be used to detect alpha-fetoprotein.

[0031] On a filter paper strip with a size of 2cm×5mm, drop a standard sample of alpha-fetoprotein with a concentration of 5ng / mL to 100ng / mL onto the...

Embodiment 2

[0033] The carboxylated single-walled carbon nanotubes were dispersed into a 2% poloxamer solution to form a dispersion with a concentration of 5 mg / mL. Mix 5 mL of single-walled carbon nanotube dispersion with 1 μL of alpha-fetoprotein antibody to form a single-walled carbon nanotube-alpha-fetoprotein complex. Single-walled carbon nanotube-alpha-fetoprotein complexes were coated onto filter paper strips with dimensions of 2 cm×5 mm. Finally, the coated paper-based sensor was placed in a 37°C oven for about 24 hours to obtain a paper-based sensor ( figure 2 ). The paper-based sensor obtained by this method can be used to detect alpha-fetoprotein.

[0034] On a filter paper strip with a size of 2cm×5mm, drop a standard sample of alpha-fetoprotein with a concentration of 5ng / mL to 100ng / mL onto the filter paper strip, and place it in an oven at 38°C for 25 hours to obtain a standard sample paper base sensor, and measure its resistance value with a multimeter, record the chan...

Embodiment 3

[0036] The carboxylated single-walled carbon nanotubes were dispersed into a 5% poloxamer solution to form a dispersion with a concentration of 7 mg / mL. Mix 6 mL of single-walled carbon nanotube dispersion with 1 μL of alpha-fetoprotein antibody to form a single-walled carbon nanotube-alpha-fetoprotein complex. Add the single-walled carbon nanotube-alpha-fetoprotein complex dropwise into the printer cartridge and wait for printing. At the same time, the detection area of ​​the paper-based sensor is designed by drawing software. The outline of the paper chip is printed first, followed by the hydrophobic areas. Finally, put the printed paper-based sensor in an oven at 37°C for about 25 hours to obtain a paper-based sensor. The paper-based sensor obtained by this method can be used to detect alpha-fetoprotein.

[0037] On a filter paper strip with a size of 2cm×5mm, drop a standard sample of alpha-fetoprotein with a concentration of 5ng / mL to 100ng / mL onto the filter paper str...

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Abstract

The invention discloses a method to quickly detect alpha-fetoprotein, comprising: applying single-wall carbon nanotube alpha-fetoprotein antibody dispersion to a paper base; measuring resistance of apaper-based carbon nanotube sensor according to resistance changes of the paper-based sensor before and after antibody and antigen combination by making use of the characteristic that alpha-fetoprotein can specifically bind with its antibody; recording changes in the resistance of the sensor for certain time so as to measure the content of a target antigen so that quick detection is achieved. Themethod herein has the advantages of good simplicity, high speed, low time consumption, good convenience and the like, and is especially suitable for early screening of cancers.

Description

technical field [0001] The invention relates to the field of sample concentration detection, in particular to a rapid detection method for alpha-fetoprotein. Background technique [0002] Primary liver cancer (primary hepatic cancer, PHC) is a malignant tumor. According to reports, it not only has a high incidence in my country, but also ranks second in the mortality rate of malignant tumors. The deterioration rate of primary liver cancer is very fast, the treatment is relatively difficult, and the treatment effect is not satisfactory. Once the disease is found, it is often in the middle or late stage. Therefore, the importance of early diagnosis of primary liver cancer is self-evident. Diagnosing the disease in time at the early stage of cancer is of great significance for improving the cure rate of cancer, prolonging the survival time of patients, and reducing the burden on families and society. [0003] PHC is easily evolved from liver disease. At present, the clinical ...

Claims

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Application Information

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IPC IPC(8): G01N27/12
CPCG01N27/12G01N27/127
Inventor 张敏张婷婷赵琳胡立傲洪晓倩杨佳圣王少男胡坪
Owner EAST CHINA UNIV OF SCI & TECH
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