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Method for quickly identifying honey varieties and adulterated honey

A technology for honey and varieties, which is applied in the field of spectral detection for identifying adulterated honey, and achieves the effects of wide application value, simple and fast operation process, and easy operation.

Active Publication Date: 2018-10-12
BEE RES INST CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The technology of using octave peaks for detection and identification of food adulteration has not been reported yet

Method used

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  • Method for quickly identifying honey varieties and adulterated honey
  • Method for quickly identifying honey varieties and adulterated honey
  • Method for quickly identifying honey varieties and adulterated honey

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0068] 1. Adopt common high fructose syrup samples of experimental samples, including 5 corn syrup samples (see the test results in Figure 9 ), 5 samples of rice syrup ( Figure 12 ), 3 samples of invert syrup-beet high fructose syrup ( Figure 10 ), 3 sucrose syrup ( image 3 ), tapioca high fructose syrup 5 ( Figure 11 ).

[0069] 2. Real honey samples for experiments: 10 pieces of rapeseed honey ( figure 2 ), 13 acacia honey ( image 3 ), jujube flower honey 10 pieces ( Figure 4 ), 8 linden honey ( Figure 5 ), Vitex 4 ( Figure 6 ), 5 goji berries ( Figure 7 ), 12 multifloral mixed honeys ( Figure 8 ).

[0070] 3. For adulterated samples, real honey is mixed with different syrups to form 10%, 20%, 30%, and 50% adulterated honey. There were 5 C4 plant syrup positive samples (identified by the national standard isotope method, among which 4 samples had a syrup content greater than 30%, and 1 sample was between 7% and 10%).

[0071] 4. Variety identification...

Embodiment 2

[0078] To verify the detection limit of the method, the sensitivity of the method was determined by adding different proportions of syrup to different honeys. Taking linden honey as an example, when 30% syrup is added, it can be accurately identified by the intensity of the emission spectrum and the octave peak. See Figure 15 , the excitation wavelength 380nm fluorescence emission spectrum of the linden honey mixed with 30% molasses (with reference to the horizontal line, when visible linden honey adds 30% molasses, its 380-650nm characteristic peak and the corresponding double frequency peak intensity are all obvious reduce).

[0079] Verification: Take 10 positive samples (confirmed as syrup adulteration samples by isotope method), and use this method to detect, 9 of them have the characteristics of syrup, and 9 of them are determined to be positive samples. The undetected positive sample was detected by isotope mass spectrometry and its adulterated amount of syrup was 13...

Embodiment 3

[0080] Embodiment 3 Manuka Variety Identification Analysis

[0081] Manuka honey is a high-value honey variety that has been identified in a number of ways. This method was also used to analyze 6 Manuka honeys from different sources. It was found that Manuka honey is different from ordinary honey varieties. When excited at 380nm, its emission wavelength ranges from 400nm to 650nm, which is higher than that of ordinary honey from 380nm to 650nm. The range of 650nm is obviously different, and its double frequency peak is far lower than the ordinary intensity. When mixed with different syrups, its emission wavelength range moves forward regularly. Mixed with 30% syrup or other ordinary honey such as rapeseed and acacia. When it is used, its emission wavelength range is 385nm-650nm, which is also obviously different from that of ordinary honey 380-650nm. Therefore, the method can effectively screen Manuka honey mixed with 30% syrup or other honey adulteration. see details Figur...

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Abstract

The invention provides a method for quickly identifying honey varieties and adulterated honey. The method comprises determining the fluorescence emission spectrum of a honey sample and comparing response values of characteristic peaks and overtones based on a blank solution as a control to determine whether the honey is adulterated, wherein the response value is represented by peak height. The method only utilizes a fluorescence spectrophotometer. The fluorescence spectrophotometer cannot be equipped with a high-pass filter or other device for filtering overtones. The method can acquire a spectrum, can be operated through simple and fast processes, realizes detection time of only 5min from pretreatment to result production and is easy to operate.

Description

technical field [0001] The invention belongs to the detection field, and in particular relates to a spectrum detection method for identifying adulterated honey. Background technique [0002] Honey is a natural sweet substance that bees collect from the nectar, secretion or honeydew of plants, combine with their own secretions, and fully brew. Because honey has important nutritional value and health care function, it has been loved by people for a long time. Honey is an extremely easy food to be adulterated. Due to the movement of interests, in recent years, some self-employed and manufacturers have mixed sugar, invert sugar, fructose, glucose, glucose high fructose syrup, fructose, etc. into honey to make adulterated honey. Fructose syrup, honey essence and other alternative raw materials are used to manufacture honey, which is put on the market in the form of natural honey, defrauding consumers for huge profits, and damaging the reputation of my country's natural honey pro...

Claims

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Application Information

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IPC IPC(8): G01N21/64
CPCG01N21/64G01N2021/6417
Inventor 薛晓锋孙明辉吴黎明尹旭升刘新迎王凯赵玲玲
Owner BEE RES INST CHINESE ACAD OF AGRI SCI
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