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General SSR molecular markers for cinnamomum and related-genera plants of cinnamomum and development method and application thereof

A technology of molecular markers and related genera, applied in the field of molecular biology, can solve the problems of difficult development of SSR markers and a small number of SSR markers, and achieve the effects of stable and efficient practicability, stable and efficient versatility, and strong practicability.

Active Publication Date: 2018-10-12
JIANGXI ACAD OF FORESTRY
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Problems solved by technology

[0006] The present invention aims at the current difficulties in developing genome SSR markers of Lauraceae plants and the lack of a small number of SSR markers, and provides general SSR molecular markers of the genus Cinnamomum and its close relatives, as well as its development method and application

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  • General SSR molecular markers for cinnamomum and related-genera plants of cinnamomum and development method and application thereof
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  • General SSR molecular markers for cinnamomum and related-genera plants of cinnamomum and development method and application thereof

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Embodiment 1

[0056] The development and application of the general microsatellite molecular markers of Cinnamomum genus and its close relatives in the present invention go through the following steps:

[0057] 1. Screening of polynucleotide microsatellite molecular marker sites in the whole genome TRF sequence of camphor tree

[0058] (1) firstly adopt TRF software to obtain the whole genome tandem repeat sequence (TRF) of camphor tree under default search conditions;

[0059] (2) Using the microsatellite analysis program (MISA) compiled by Perl language and the online microsatellite site analysis software (Micr osatellite Repeats Finder), the microsatellite site screening was carried out to the TRF of the camphor tree genome under the default search conditions, and the camphor tree genome was obtained Microsatellite sequence;

[0060] (3) Based on the principle that the longer the repeated nucleotide sequence, the higher the polymorphism, the microsatellite site of the single nucleotide ...

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Abstract

The invention discloses general SSR molecular markers for cinnamomum and related-genera plants of the cinnamomum and a development method and application thereof. The nucleotide sequences of the general SSR molecular markers for the cinnamomum and the related-genera plants of the cinnamomum are shown in sequence tables SEQ ID NO.1-SEQ ID NO.8. A group of the polymorphic microsatellite markers andamplification primers thereof can be directly applied to intraspecific and interspecific structures in the cinnamomum and the related-genera plants of the cinnamomum, have good universality and practicability, and are stable and efficient. As the genetic background is not clear, the cinnamomum and the related-genera plants are limited by the EST sequence quantitative limiting, EDT-SSR molecular markers cannot be difficult to largely develop at the present stage, and the problem that the EDT-SSR molecular markers cannot be difficult to largely develop is solved through the general microsatellite molecular markers developed with the method.

Description

Technical field: [0001] The invention belongs to the field of molecular biology, and in particular relates to a universal SSR molecular marker for plants of the genus Cinnamomum and its close relatives, a development method and application thereof. Background technique: [0002] Microsatellite (microsatellite), also known as simple sequence repeat (SSR), refers to a class of repeating sequences consisting of several nucleotide pairs (called core sequences, generally 1 to 6 bps) throughout the genome of organisms. Simple tandem repeats of units. The number of repeats is highly variable among different genotypes of the same species, but the two ends of this repeat are relatively conserved single-copy sequences. [0003] Compared with other marker technologies, SSR molecular markers have the following characteristics: (1) The sequences at both ends of each microsatellite DNA are mostly relatively conserved single-copy sequences, especially among species with close relatives, a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6895C12Q1/686C12N15/11
CPCC12Q1/686C12Q1/6895C12Q2600/156C12Q2525/151
Inventor 伍艳芳徐海宁刘新亮郑永杰李江杨海宽汪信东章挺王建
Owner JIANGXI ACAD OF FORESTRY
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