Giant Panda Microsatellite Polymorphic Sites, Identification Methods and Primer Sequences

Abstract

The invention discloses panda microsatellite polymorphic loci, an identification method and primer sequences. 21 pairs of the specific primer sequences of the panda microsatellite polymorphic loci that are screened out are shown in SEQ ID NO.1 to SEQ ID NO.42. The identification method for the panda microsatellite polymorphic loci comprises the steps as follows: screening out the microsatellite loci and designing high-quality primers based on a panda genome; comparing read data of panda genome sequencing with a panda reference genome to identify INDEL, and screening out the microsatellite lociwith polymorphism and the high-quality primers by combining INDEL identification results with screening of the microsatellite loci. The 21 pairs of microsatellite primers are all polymorphic sites and have higher resolution capacity. Individual identification and parent-child relationship identification of pandas can be performed economically, quickly and correctly by the microsatellite primers,family pedigree reconstruction and information improvement are performed on panda populations, and data support is provided for formulation of scientific panda protection programs.

Description

technical field [0001] The invention belongs to the field of giant panda microsatellite polymorphic site identification, and specifically relates to a giant panda satellite polymorphic site, an identification method and a specific primer sequence. [0002] technical background [0003] The giant panda is a unique endangered species in my country and is a first-class national protected animal. The giant panda is an ancient animal that was once widely distributed in vast areas of my country and Southeast Asia. However, with the severe destruction of the panda habitat by human civilization, the giant panda, a species that was once widely distributed, had to retreat to the present The three provinces of Sichuan, Shaanxi and Gansu are distributed in six mountain systems including Qinling Mountains, Minshan Mountains, Qionglai Mountains, Daxiangling Mountains, Xiaoxiangling Mountains and Liangshan Mountains, and each has a unique evolutionary history. Since the end of the 1970s, re...

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Problems solved by technology

However, the previous method of screening microsatellites using species reference genomes has two defects: 1. The specificity of primer design is poor, which is prone to non-specific amplification; Spending more time and cost for further screening

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