Method for obtaining new rice germplasm through utilizing fargesia DNA (deoxyribonucleic acid) to combine with pollen tube channel

Abstract

The invention belongs to plants or methods for obtaining new plants, and discloses a method for obtaining new rice germplasm through utilizing fargesia DNA (deoxyribonucleic acid) to combine with a pollen tube channel in the technical field of plant regeneration through tissue culture technology. The method comprises the following steps of treating a rice variety Enhui 142 to generate mutation byutilizing a method by which grass family arundhoriaalpha whole genome DNA combines with the pollen tube channel, screening single plants of which the phenotypes are obviously different from those of reference samples in 318 seedlings generated from 450 glumous flowers to perform continuous selfing to obtain 13 mutant strains with stable phenotypes; analyzing the mutant strains and the reference samples by utilizing 48 pairs of SSR molecular markers, and discovering that the genetic background between the mutant strains and the reference samples and the genetic background between the mutant strains both have differences; enabling mutant materials and the reference samples to be respectively subjected to hybridization and matching with photo-thermo-sensitive genic male sterile lines. The method for utilizing the fargesia DNA to combine with the pollen tube channel can be used for creating new germplasm for rice breeding, and provides a new approach for the germplasm resource innovation of rice.

Description

technical field [0001] The invention belongs to the plant or method for obtaining new plants; the technical field of plant regeneration through tissue culture technology, and particularly relates to a method for obtaining new rice germplasm by using arrow bamboo DNA combined with pollen tube passages. Background technique [0002] At present, the existing technologies commonly used in the industry are as follows: The pollen tube passage method established by Zhou Guangyu in 1983 can be applied to any flowering plant. Its basic principle is to use the elongated pollen tube after pollination to introduce foreign DNA along the nucellus channel into the embryo sac, and the transformation does not yet have a normal cell wall. egg, zygote or early embryonic cells. For the first time, Duan Xiaolan et al. introduced the whole genome DNA of purple rice into green rice through the pollen tube channeling method, and obtained plants that can stably inherit purple traits in the offsprin...

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Problems solved by technology

[0003] In summary, the problems in the prior art are: At present, there are no methods and studies on the hybridization of plants from different subfamilies of rice and bamboo; and the pollen tube passage method has great advantages in innovating plant genetic breeding materials, but many experiments lack clear molecular evidence and cannot prove source of variation

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