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Application of synovial fluid alpha defensin in detection and diagnostic kit for prosthetic joint infection

A technology of defensin and joint fluid, which is applied in the field of medical diagnosis, can solve the problems of high price, low specificity, and high rise, and achieve good application and industrialization prospects, good anti-interference ability, and strong detection sensitivity.

Inactive Publication Date: 2018-09-28
THE FIRST AFFILIATED HOSPITAL OF FUJIAN MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

This patented technology provides an improved way to detect if there're any substances that may cause inflammation or damage during medical procedures such as hip replacement surgery. It uses scleroid beta protein from human placenta tissue to identify pneumonia caused by bacteria called pseudomonas aeruginosa. These markers have been shown to be highly sensitive but also very stable against interfering agents like antibiotics used before implantations. They could help guide clinicians towards appropriate treatment strategies based on their understanding of these indicators.

Problems solved by technology

This patented technical problem addressed in this patents relates to improving the accuracy and reliability of identifying pneumonia caused by implanted prostheses such as hip replacements without causing any negative side effects that may affect patient outcomes during reconstructive surgerys. Current techniques like histological analysis require expensive equipment and time-consumings, making them challenging to apply outside hospitation settings due to lack of affordable options.

Method used

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  • Application of synovial fluid alpha defensin in detection and diagnostic kit for prosthetic joint infection
  • Application of synovial fluid alpha defensin in detection and diagnostic kit for prosthetic joint infection
  • Application of synovial fluid alpha defensin in detection and diagnostic kit for prosthetic joint infection

Examples

Experimental program
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Effect test

Embodiment 1

[0023] Example 1 Preparation of joint fluid α-defensin immunofluorescence test strip

[0024] 1. Antibody preparation

[0025] 1) Prepare anti-α defensin antibody and goat anti-mouse IgG antibody by genetic engineering method; the antibody can be prepared by the laboratory itself, or can be entrusted to a commercial company.

[0026] 2) Select time-resolved fluorescent latex particles, and screen them in terms of particle size and surface groups; for example, polymer polystyrene particles with a diameter of 100nm, embedded with europium-chromium compounds, have the characteristics of time-resolved fluorescence, and the surface of the particles It has a high-density functional group-COOH (the above-mentioned particles and their parameters are not the limiting conditions of the present invention); it can be purchased directly from commercial companies.

[0027] 3) Fluorescent particle-labeled antibody, using the covalent coating method: take 25 mg of particles and wash them twi...

Embodiment 2

[0036] The collection of embodiment 2 joint fluid specimen and clinical data

[0037] Approved by the Ethics Committee of the First Affiliated Hospital of Fujian Medical University, the synovial fluid of patients re-admitted for various reasons after artificial joint replacement was collected. At the same time, detailed clinical data of patients were collected. According to the diagnostic criteria of the American Musculoskeletal Infection Society (MSIS), they were divided into an infection group of 22 cases and a non-infection group of 23 cases. After centrifuging the joint fluid, the supernatant was collected and frozen for future use. Before use, thaw the stored joint fluid and stabilize at room temperature for later use.

Embodiment 3

[0038] Example 3 Detection of α-defensins in synovial fluid by ELISA method

[0039] 1) Take 1 mL of joint fluid and centrifuge at 8000rpm for 4 minutes to separate the supernatant and cells of the joint fluid, absorb the supernatant, transfer it to an EP tube, and store it in a -80°C refrigerator for testing.

[0040] 2) According to the ELISA kit instruction method, use the ELISA kit (CUSABIO company) to detect α-defensin in the supernatant, detect the content of α-defensin in the joint fluid of patients in the infection group and non-infection group, and perform statistical analysis.

[0041] 3) The clinical data of 45 patients were collected, and the threshold value for diagnosing PJI was determined according to the diagnostic criteria of MSIS.

[0042] 4) The experimental results are as follows: the median expression of α-defensin in the infected group was 321.2ng / mL (P25=226.9ng / mL, P75=450.5ng / mL), and the median expression of α-defensin in the non-infected group It wa...

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Abstract

The invention relates to an application of synovial fluid alpha defensin in detection and a diagnostic kit for prosthetic joint infection. Joint replacement is an effective treatment mode for treatingjoint disease at a terminal stage, and periprosthetic joint infection is a severe complication after joint replacement. The synovial fluid alpha defensin has high sensitivity and specificity for diagnosing the prosthetic joint infection. According to the invention, fluorescence particles are taken as a maker, and the diagnostic kit (a test paper strip) for the synovial fluid alpha defensin is prepared through an immunochromatography method. By using an immunofluorescence quantitative analyzer, quantitative detection and instant detection on a synovial fluid specimen can be carried out, and diagnosis of the prosthetic joint infection is realized. A detection reagent and the diagnostic kit have the advantages of simple usage, sensitive reaction, and high diagnosis accuracy.

Description

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Claims

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Application Information

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Owner THE FIRST AFFILIATED HOSPITAL OF FUJIAN MEDICAL UNIV
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