Tandem mass spectrometry method for trace substance

Abstract

A tandem mass spectrometry method for a trace substance adopts an ion trap mass analyzer or a quadrupole rod and ion trap combined mass analyzer. A quadrupole can realize simultaneous selective transmission of multi-target parent ions by coupling a low-voltage auxiliary alternating current signal, free from a multi-frequency window in a frequency spectrum, on a high-voltage radiofrequency, and anion trap can realize simultaneous selective isolation of the multi-target parent ions; after the ion trap binds selected ions, the multi-frequency low-voltage auxiliary alternating current signal is used together with a buffer gas to complete the simultaneous fragmentation of the multiple parent ions; then a single-frequency low-voltage auxiliary alternating current signal is coupled to the ion trap, and the radiofrequency voltage is scanned to realize the resonance excitation mass spectrometry analysis of corresponding fragment ions; and then the obtained mass spectrogram is compared with thetandem mass spectrogram database corresponding to each parent ion in order to resolve the tandem mass spectrogram corresponding to each target parent ion. The method can realize simultaneous tandem mass spectrometry analysis of multiple ions by one-shot injection without noise ion interference, and has a good application prospect in the field of trace sample analysis.

Description

technical field [0001] The invention relates to a method suitable for performing serial mass spectrometry analysis on trace substances, and belongs to the field of biochemical substance detection using mass spectrometers. Background technique [0002] Mass spectrometry is a universal chemical analysis method with high specificity and high sensitivity, which has been widely used in various fields such as life sciences, medicine and health, environmental monitoring, and public safety. Conventional mass spectrometry converts substances into ions, and obtains the mass-to-charge ratio information of ions through analysis; while tandem mass spectrometry helps to characterize and analyze the material structure by breaking ions and analyzing the information of their fragment ions, so it has Powerful function. Among various mass spectrometers, quadrupole mass analyzer mass spectrometry has become an important branch of mass spectrometry instruments due to its powerful tandem mass sp...

AI Technical Summary

Problems solved by technology

[0006] The purpose of the present invention is to overcome the technical defects in the analysis of trace samples caused by the collision-induced dissociation of parent ions in one or a small range of continuous mass-to-charge ratios in the existing tandem mass spectrometry method, Provides a method for collision-induced dissociation (CID) tandem mass spectrometry analysis of multiple target precursor ions in one injection in an ion trap mass analyzer

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