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Cell, tissue and microorganism preservation solution, preparation method and application

A technology for preserving liquid and microorganisms, applied in the field of medical systems, can solve the problems of high price, high transportation environment requirements, and short preservation time, and achieve the effects of low price, low transportation environment requirements, and long preservation time.

Inactive Publication Date: 2018-09-28
江西业力医疗器械有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Therefore, the technical problem to be solved by the present invention is to overcome the short storage time of cells, tissues and microorganisms in the prior art, toxic solvents, relatively expensive prices, and high requirements on the transportation environment, thereby providing a cell , tissue and microbial preservation solution

Method used

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  • Cell, tissue and microorganism preservation solution, preparation method and application
  • Cell, tissue and microorganism preservation solution, preparation method and application
  • Cell, tissue and microorganism preservation solution, preparation method and application

Examples

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Effect test

Embodiment 1

[0038] The weight ratio of each component of the cell, tissue and microorganism preservation solution of the present embodiment is as follows:

[0039] 25 parts of potassium chloride, 25 parts of sodium chloride, 5 parts of disodium hydrogen phosphate, 500 parts of glycerin, 2 parts of cetyltrimethylammonium chloride, 10000 parts of distilled water, appropriate amount of peacock blue pigment;

[0040] The cell, tissue and microorganism preservation solution of the present embodiment are prepared according to steps:

[0041] (1) get each raw material by weight;

[0042] (2) Mix potassium chloride, sodium chloride, glycerin, and cetyltrimethylammonium chloride evenly, add water to dissolve each component, then add distilled water to a certain volume, and adjust with disodium hydrogen phosphate To pH 6.0-7.0, then add peacock blue pigment, mix and filter to obtain a clear and transparent mixed solution;

[0043] The preservation effect of the cell, tissue and microorganism pres...

Embodiment 2

[0045] The weight ratio of each component of the cell, tissue and microorganism preservation solution of the present embodiment is as follows:

[0046] 10 parts of glycerin, 1 part of trisodium phosphate, 1 part of phenol, 5 parts of dithiothreitol, 100 parts of distilled water, appropriate amount of emerald green pigment.

[0047] The cell, tissue and microorganism preservation solution of the present embodiment are prepared according to steps:

[0048] (1) get each raw material by weight;

[0049] (2) Add distilled water to glycerin, phenol, and dithiothreitol to dissolve each component, then add distilled water to a certain volume, adjust the pH to 6.0-7.0 with trisodium phosphate, add emerald green pigment, and obtain a mixed solution.

[0050] The preservation effect of the cell, tissue and microorganism preservation solution of the present embodiment can be referred to Figure 5 to Figure 8 .

Embodiment 3

[0052] The weight ratio of each component of the cell, tissue and microorganism preservation solution of the present embodiment is as follows:

[0053] 40 parts of sodium chloride, 40 parts of potassium chloride, 10 parts of trisodium phosphate, 50 parts of disodium edetate, 10 parts of phenol, 50 parts of dithiothreitol, and 50 parts of bromocetyl trimethylamine , 100 parts of glycerol, 50 parts of octadecyltrimethylammonium chloride, 5 parts of disodium hydrogen phosphate, 1000 parts of distilled water, and an appropriate amount of amaranth.

[0054] The cell, tissue and microorganism preservation solution of the present embodiment are prepared according to steps:

[0055] (1) get each raw material by weight;

[0056] (2) Sodium chloride, potassium chloride, trisodium phosphate, disodium edetate, phenol, dithiothreitol, cetyl trimethylamine bromide, glycerol, octadecyl trimethylamine Mix methylammonium chloride and disodium hydrogen phosphate evenly, add distilled water to d...

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Abstract

The invention discloses a cell, tissue and microorganism preservation solution, a preparation method and application, and belongs to the field of medical systems. The cell, tissue and microorganism preservation solution comprises the raw materials of one or more of potassium chloride, sodium chloride, disodium hydrogen phosphate, trisodium phosphate, glycerine, phenol, dithiothreitol, ethylene diamine tetraacetic aci, cetyltrimethyl ammonium bromide, cetyltrimethyl ammonium chloride, hexadecyl trimethyl ammonium chloride, Hexadecyl trimethyl ammonium chloride, pigment and distilled water. Thecell, tissue and microorganism preservation solution is lower in price and has lower requirements on transportation environment.

Description

technical field [0001] The invention relates to the field of medical systems, in particular to a cell, tissue and microorganism preservation solution, preparation method and application. Background technique [0002] After the cells or tissues are taken out from the living body of the human body or animal, before they are put into the microscope for detection, there are many, many uncontrollable factors in the process of storage and transportation, which cause the cells, tissues and microorganisms to be oxidized, enzymatically hydrolyzed, etc. detection. [0003] At present, the cell or tissue preservation solution is mainly hypertonic saline solution or potassium dichromate solution. In addition, formalin solution is also used as the preservation solution, but formalin itself is toxic, and it is easy to cause adverse damage to the testing personnel for long-term use. . In addition, there is an imported preservation solution, which does not contain toxic reagents and has a...

Claims

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Application Information

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IPC IPC(8): C12N1/04A01N1/02A01N1/00
CPCA01N1/00A01N1/021C12N1/04
Inventor 吕正和陈永洪王金钱余尧毅田成鑫杨海敏
Owner 江西业力医疗器械有限公司
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