A kind of astaxanthin-collagen coupling substance and its preparation method and application

A technology of collagen and astaxanthin, applied in the field of medicine, can solve problems such as the unstable original molecular form of astaxanthin, and achieve the effects of preventing and treating skin photoaging, improving vitality, and reducing skin thickness

Active Publication Date: 2019-12-24
GUANGDONG PHARMA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The fundamental reason is that astaxanthin still exists in an unstable original molecular form in various existing applications, and physical methods cannot essentially solve the limitations of astaxanthin applications

Method used

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  • A kind of astaxanthin-collagen coupling substance and its preparation method and application
  • A kind of astaxanthin-collagen coupling substance and its preparation method and application
  • A kind of astaxanthin-collagen coupling substance and its preparation method and application

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preparation example Construction

[0031] A preparation method of an astaxanthin-collagen conjugate, comprising the following steps:

[0032] 1) Mixing astaxanthin-rich raw materials with a water-soluble organic solvent to remove insoluble components; dissolving collagen in water;

[0033] 2) Add and mix the above two solutions dropwise under acidic conditions at a temperature of 4-60°C to dissolve the collagen and astaxanthin in the reaction solution, and stir to react in the dark;

[0034] 3) Separating and purifying the astaxanthin-collagen conjugate produced in the above reaction solution to obtain the astaxanthin-collagen conjugate.

[0035] Preferably, in step 2), the acidic condition refers to a pH of 3.0-6.0, more preferably 4.5-5.5, most preferably 5.0.

[0036] Preferably, the temperature in step 2) is preferably 4-45°C, most preferably 37°C.

[0037] Preferably, in step 2), the molar ratio of astaxanthin to collagen added in the reaction solution is 1:1˜10:1.

[0038] Preferably, in step 2), the v...

Embodiment 1

[0054] Embodiment 1 A kind of preparation method of astaxanthin-collagen coupling substance

[0055]The (3S, 3S') astaxanthin monomer derived from Haematococcus pluvialis was dissolved in ethanol, the insoluble components were removed by centrifugation, and the hydrolyzed collagen with an average molecular weight of 15600 was dissolved in water. Adjust the pH of the above two solutions to 5.0, add dropwise and mix at 25°C, the molar ratio of astaxanthin and collagen in the mixed solution is 10:1, and the volume ratio of ethanol and water in the mixed solution is 2:1 , after stirring for 2 hours in the dark, add saturated ammonium sulfate solution to the reaction solution to precipitate the protein, collect the protein precipitate, freeze-dry and store in the dark.

[0056] After detection, the ultraviolet characteristic absorption peak of the astaxanthin-collagen coupling product prepared above is near 418nm (see figure 1 ), has a characteristic ultraviolet absorption peak di...

Embodiment 2

[0057] Embodiment 2 A kind of preparation method of astaxanthin-collagen coupling substance

[0058] Mix Haematococcus pluvialis powder (3% w / w astaxanthin content) with acetone, stir, shake at 4°C for 40 min, centrifuge (4000 r / min, 10 min), and take the supernatant. Hydrolyzed collagen with a number average molecular weight of 5000 was dissolved in water. Adjust the pH of the above solution to 4.0, add dropwise and mix at 4°C, the molar ratio of astaxanthin and collagen added after mixing is 5:1, the volume ratio of acetone and water is 1:1, and react in dark place after stirring for 1 hour , continue to add cold acetone dropwise to the reaction solution until the protein precipitates, collect the protein precipitates, freeze-dry and store in the dark at low temperature.

[0059] After testing, the ultraviolet characteristic absorption peak of the astaxanthin-collagen coupling product prepared above is around 390nm, which is different from the ultraviolet characteristic abs...

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Abstract

The invention discloses an astaxanthin-collagen conjugate as well as a preparation method and application thereof. According to the method, raw materials containing rich astaxanthin are mixed with water-soluble organic solvents; insoluble ingredients are removed; the collagen is dissolved by water; the two solutions are dripped and mixed under the acidic condition at the temperature of 4 to 60 DEGC, so that the collagen and the astaxanthin are dissolved in the mixed reaction solution; the light-free stirring reaction is performed. The astaxanthin-collagen conjugate generated in the reaction liquid is separated and purified to obtain a finished product.

Description

technical field [0001] The invention belongs to the technical field of medicine, and in particular relates to the preparation of an astaxanthin-collagen coupling product and its application in preventing and treating skin photoaging. Background technique [0002] Skin photoaging is the cumulative damage caused by long-term exposure to ultraviolet radiation. Excessive ultraviolet radiation will degrade skin collagen, hyaluronic acid and elastin, resulting in changes in skin appearance. In severe cases, it can lead to basal cell carcinoma, melanoma and Formation of neoplastic diseases such as squamous cell carcinoma. At present, the prevention and treatment of photoaging has become a research hotspot in the fields of medicine, cosmetics and biomaterials, and the development of safe and effective photoaging prevention and treatment products is an important direction of current related industry research. [0003] Astaxanthin (3,3′-dihydroxy-4,4′-diketo-β-carotene, Astaxanthin, ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K47/64A61K31/122A61K8/35A61K8/65A61P17/16A61P17/18A61Q19/08
CPCA61K8/35A61K8/65A61K31/122A61K47/6435A61P17/16A61P17/18A61Q19/08
Inventor 刘涛李芸王鑫周婉尹辉戴良成金小宝
Owner GUANGDONG PHARMA UNIV
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