High-flux identification method for purity of Xinjiang thick-peel muskmelon Huangpi 9818 hybrid variety based on SSR molecular marker

Abstract

The invention discloses a high-flux identification method for purity of Xinjiang thick-peel muskmelon Huangpi 9818 hybrid variety based on an SSR molecular marker. The method comprises the following steps: extracting DNA by adopting an alkaline lysis method, placing seeds in a 96-pore PCR plate, adding a solution buffer A, performing the hot bath for 10 minutes at 95 DEG C in a PCR instrument, then adding a buffer B, uniformly mixing, wherein the obtained solution can be used for PCR amplification. A polymorphism SSR primer is screened by utilizing parents, F1 is used for verification, 8 pairsof parental complementary type primers are screened. The sequencing and combination are performed according to the size of a PCR amplification product fragment, the PCR amplification can be separately performed, the PCR products are mixed, the electrophoresis is performed in a sheet of polyacrylamide gel, after the silver staining, a strip is read, and then the purity of the hybrid variety is identified. The identification result is basically consistent with the traditional purity identification result of the field hybrid variety. By adopting the method, the muskmelon Huangpin 9818 hybrid variety purity can be rapidly and accurately identified by virtue of high flux, the cost is low, and the application prospect is wide.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to a method for high-throughput identification of the purity of Xinjiang muskmelon "Huangpi 9818" hybrids based on SSR molecular markers. Background technique [0002] Melon (Cucumis melo L., 2n=2x=24) belongs to Cucurbitaceae Cucurbitaceae. It is a worldwide fruit with unique flavor and is widely cultivated at home and abroad. In China, melons are usually divided into thin-skinned melons and thick-skinned melons. Thin-skinned melons are mainly grown in eastern provinces, while thick-skinned melons are mainly grown in Xinjiang and other northwest regions. However, with the development of the "south-to-east" ecological breeding work carried out by academician Wu Mingzhu's team, the planting range of thick-skinned melon has continued to expand, and it has gradually become a characteristic economic crop that helps the adjustment of agricultural industrial structure and farmers' income gener...

AI Technical Summary

Problems solved by technology

In the application process, only a single marker is used for identification, the accuracy is not enough, and it is easy to cause misjudgment

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