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The establishment method and fingerprint spectrum of Ji Chuanjian

A technique of fingerprinting and establishing a method, which is applied in the field of quality standard detection of traditional Chinese medicine compound granules, can solve the problems of the composition quality status, few indicators, and many components of Duojichuan decoction, and achieve the control of the quality of Jichuan decoction preparations and convenient operation , the objective effect of the conclusion

Active Publication Date: 2020-05-05
CHINA RESOURCES SANJIU MEDICAL & PHARMA +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Therefore, the technical problem to be solved in the present invention is to overcome the traditional Chinese medicine compound preparations in the prior art, which basically only have one or two index components in content control, single quality control, few indexes, and it is difficult to fully reflect that there are many flavors and many components. , the defects of the quality status of the Jichuan fried composition with many targets, thereby providing the method for establishing the fingerprint of Jichuan fried and the fingerprint

Method used

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  • The establishment method and fingerprint spectrum of Ji Chuanjian
  • The establishment method and fingerprint spectrum of Ji Chuanjian
  • The establishment method and fingerprint spectrum of Ji Chuanjian

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Example 1. Preparation of Jichuan Fried Composition

[0042] According to the following proportions, take 625g of Cistanche deserticola; 1000g of angelica; 500g of Achyranthes bidentata; 375g of Alisma alisma; Filtrate, concentrate the filtrate to a clear paste with a relative density of 1.20-1.25 (60° C.), add an appropriate amount of maltodextrin, mix well, dry, and granulate to make 1000 g of Jichuan decoction composition.

Embodiment 2

[0043] Embodiment 2. Establish Jichuan Fried Fingerprint

[0044] Instruments and reagents

[0045] High performance liquid chromatography: Agilent 1100 Series;

[0046] Electronic analytical balance: Germany Sartorius BT25s and BS124s

[0047] Ultrasonic cleaning machine: Beijing Ultrasonic Instrument Co., Ltd.

[0048] Chromatographic column: Agilent eclipse XDB C18 column (5μm, 4.6mm×250mm);

[0049] Reagents: Acetonitrile is chromatographically pure, water is ultrapure water; other reagents are analytically pure.

[0050] Reagent: Jichuanjian Granules (batch number: 1602001J).

[0051] (1) Chromatographic conditions

[0052] Use octadecylsilane bonded silica gel as filler (column length 250mm, inner diameter 4.6mm, particle size 5μm); use acetonitrile as mobile phase A, 0.1% phosphoric acid solution as mobile phase B, and carry out as specified in the following table Gradient elution. The flow rate is 1.0ml per minute; the column temperature is 30°C; the detection w...

Embodiment 3

[0064] Embodiment 3. Methodological investigation of Ji Chuan fried fingerprint chromatogram

[0065] Precision experiment

[0066] Take the batch number 1602001J and prepare 1 part of the test solution according to the preparation method of the test solution. The precision is continuously injected 6 times, 10 μl each time. The similarity calculation results are shown in Table 2. The results showed that the similarity of each chromatographic peak reached more than 0.95, which met the requirements of the fingerprint, indicating that the precision of the instrument was good.

[0067] Table 2 Precision inspection results

[0068]

[0069] Summary: It can be seen from the experimental results of the similarity of precision fingerprints that the similarity is 0.998-1.0, and the experimental equipment is feasible.

[0070] Stability test

[0071] Prepare 1 part of need testing solution according to the preparation method of need testing solution, respectively in 0 hour, 1 hou...

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Abstract

The invention discloses an establishing method of a jichuan decoction fingerprint atlas and a fingerprint atlas, wherein the method and the fingerprint atlas belong to the field of traditional Chinesemedicine analysis. According to the method of the invention, the jichuan decoction fingerprint atlas is established by means of high-efficiency liquid chromatography. Chromatogram conditions are characterized in that a chromatographic column is an octadecylsilane chemically bonded silicacharomatographic column; the detecting wavelength is 320nm; the flow speed is 0.9-1.1ml / min; the column temperature is 25-35 DEG C; a theoretical board number is not lower than 5000 when calculated according to a ferulic acid peak; acetonitrile (A)-0.1% phosphoric acid solution (B) is used as a mobile phase, and gradient elution is performed according to a sequence which is shown in the description. The fingerprint atlas comprehensively reflects quality information of the jichuan decoction, thereby more comprehensively and effectively controlling the quality of a jichuan decoction product.

Description

technical field [0001] The invention belongs to the field of traditional Chinese medicine analysis, and relates to a quality standard detection method for traditional Chinese medicine compound granules. Background technique [0002] Traditional Chinese medicine decoction is the mainstream of clinical medicine, but because of decoction and inconvenient to carry, the quality of the decoction prepared by decoction often varies greatly depending on the person and the decoction utensils. The use of modern science and technology to develop traditional Chinese medicine preparations that are easy to carry and easy to take is not only the inheritance of traditional Chinese medicine, but also a better promotion of classic famous prescriptions. Clinical application. The preparation process of granules is relatively simple, easy to shape, easy to take and carry, and the form of taking is most consistent with traditional decoctions, and can maintain the characteristics of traditional de...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/88
CPCG01N30/88G01N2030/8809
Inventor 林丽娜郭小芳刘志刚陈周全谭沛马鹏岗曾祥腾高云佳屠鹏飞
Owner CHINA RESOURCES SANJIU MEDICAL & PHARMA
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