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A kind of SNP marker related to high alkalinity resistance of Litopenaeus vannamei, detection primer and its application

A technology for resistance-related and detection primers, applied in the field of detection primers and SNP markers, can solve the problems of long cycle, low efficiency, instability, etc.

Active Publication Date: 2020-09-29
SOUTH CHINA SEA INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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Problems solved by technology

[0004] The traditional breeding method is completely dependent on phenotype, which has disadvantages such as long cycle, low efficiency and instability

Method used

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  • A kind of SNP marker related to high alkalinity resistance of Litopenaeus vannamei, detection primer and its application
  • A kind of SNP marker related to high alkalinity resistance of Litopenaeus vannamei, detection primer and its application
  • A kind of SNP marker related to high alkalinity resistance of Litopenaeus vannamei, detection primer and its application

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Embodiment

[0019] Under the stress condition of pH 9.6±0.2, a high alkalinity tolerance test was carried out on 642 offspring from the hybridization of "Zhengda" and "Zhongke No. group, Litopenaeus vannamei still survived after 24 hours as the high pH tolerance group.

[0020] During the experiment, observations were made every half an hour, and the dead individuals were stored in 95% alcohol in time. The high pH sensitive group and the high pH tolerant group were extracted from shrimp genomic DNA according to the Tiangen Marine Animal Tissue Genome Extraction Kit (TIANGEN, Tiangen Biochemical Technology Co., Ltd.).

[0021] Using the detection primers POLR2A-F (5'-AGCACCTCAAGCAGCACAT-3', as shown in SEQ ID NO.2) and POLR2A-R (5'-GTCAGCCAGTAGCATTACATGCC-3', as shown in SEQ ID NO.3) disclosed in the present invention, Perform PCR detection on shrimp genomic DNA in the high pH sensitive group and high pH tolerant group. The reaction program is: 95°C pre-denaturation for 4 minutes, (95°C d...

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Abstract

The invention discloses an SNP (single nucleotide polymorphism) marker associated with high-basicity resistance of litopenaeus vannamei, a detection primer of the SNP marker and application of the SNPmarker. The SNP marker disclosed by the invention is positioned at the 237th basic group position of an SEQ ID-POLR2A sequence; the basic group of a variation point is A or C; and when the genotype of the variation point is AC, the survival rate of the litopenaeus vannamei under the high-basicity environment is obviously lower. The invention also discloses primer combination for detecting the variation point and a breeding application method thereof. In the stress-resistant breeding work of the litopenaeus vannamei, the backup parent populations can be screened by the method disclosed by theinvention, the litopenaeus vannamei individuals which are sensitive to the high-basicity environment pressure can be eliminated as soon as possible, and high-basicity stress-resistant breeding of thelitopenaeus vannamei can be benefited.

Description

technical field [0001] The invention belongs to the field of aquatic biology technology and molecular breeding, and specifically relates to a SNP marker related to the high alkalinity resistance of Litopenaeus vannamei, a detection primer and an application thereof. Background technique [0002] Litopaeus vannamei (Litopenaeus vannamei, commonly known as vannamei) is native to the Pacific coast of Central and South America. Litopenaeus vannamei was introduced to my country from the United States in 1988, and large-scale farming began in 1998. Now it is the most important shrimp farming species in my country. . As of 2016, the annual output of aquaculture in my country has exceeded 1.6 million tons, the annual output value has exceeded 60 billion yuan, and the annual demand for seedlings has exceeded 500 billion. The breeding of improved species is of great significance to the aquaculture industry of Litopenaeus vannamei. [0003] Because Litopenaeus vannamei has strong envir...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6888C12N15/11
CPCC12Q1/6888C12Q2600/124C12Q2600/156
Inventor 黄文任春华程楚杭罗鹏陈廷胡超群
Owner SOUTH CHINA SEA INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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