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Application of UCA1 as target site to preparation of drugs for treating hyperpigmentation dermatoses

A skin disease, target site technology, applied in the field of biomedicine

Active Publication Date: 2018-08-28
THE THIRD XIANGYA HOSPITAL OF CENT SOUTH UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, there is no research report on the effect and mechanism of UCA1 on the melanogenesis function of melanocytes

Method used

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  • Application of UCA1 as target site to preparation of drugs for treating hyperpigmentation dermatoses
  • Application of UCA1 as target site to preparation of drugs for treating hyperpigmentation dermatoses
  • Application of UCA1 as target site to preparation of drugs for treating hyperpigmentation dermatoses

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Experimental program
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Embodiment Construction

[0018] The present invention will be further described below in conjunction with the accompanying drawings and embodiments.

[0019] experimental method:

[0020] 1. Main materials

[0021] A lentiviral overexpression vector was constructed by Shanghai Jikai Gene. Three lentiviral knockdown vectors were constructed by Suzhou Gemma Gene.

[0022] 2. Cell culture:

[0023] At 37°C, 5% CO 2 Immortalized human melanocytes (PIG1 cells) and primary human skin melanocytes (MC) were cultured in 5% fetal bovine serum (Biological Industries, Israel), 1% double antibody (Gibco) and 1% HMGS (# S0025, Gibco) 254 (#M-254-500, Gibco) medium. Melanoma cells SK-MEL-28 and A375 cells were cultured in DMEM (Hyclone) medium with 10% fetal bovine serum and 1% double antibody (Gibco).

[0024] 3. Determination of melanin content

[0025] MC, PIG1, SK-MEL-28, A375 cells (2.5×10 5 cells per 35mm Petri dish), trypsinized, centrifuged, collected cells, heated 100ul 1mol / L NaOH in a 70°C water b...

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Abstract

According to the experimental results of the invention, UCA1 has a negative regulatory effect on melanogenesis of melanocytes, and the expression level of melanogenesis-related genes is decreased after overexpression of UCA1 in melanocytes, and is obviously enhanced after UCA1 is knocked out, and UCA1 negatively regulates melanogenesis mainly through a cAMP / PKA / CREB pathway. The results of this study suggest that UCA1 is a potential therapeutic target for regulating melanogenesis in melanocytes, and the development of drugs or treatments that regulate the expression level of UCA1 is expected to bring new hope to the treatment of hyperpigmentation dermatoses.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, and specifically relates to the application of UCA1 as a target site in the preparation of drugs for treating hyperpigmentation skin diseases. Background technique [0002] Hyperpigmentation dermatosis is caused by the increase of melanin produced by skin melanocytes caused by various physical, chemical, physiological and other factors. It is a large class of common diseases in dermatology. Although most hyperpigmentation skin diseases will not have a great impact on physical health, skin pigmentation, especially facial skin pigmentation, seriously affects the appearance of patients, and has a great impact on patients' mental health. A lot of mental pressure even affects the patient's work, study and life. And in recent years, people have higher and higher requirements for their own appearance, and the rate of visits of patients with hyperpigmentation skin diseases in dermatology clinics is ...

Claims

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Application Information

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IPC IPC(8): A61K31/7105A61P17/00
CPCA61K31/7105
Inventor 曾庆海陈静黄进华裴诗谣雷厉鲁建云康健夏芳康丽阳
Owner THE THIRD XIANGYA HOSPITAL OF CENT SOUTH UNIV
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