Preparation method of skin-care earthworm active polypeptide extracting solution
A technology of active polypeptides and extracts, applied in skin care preparations, skin diseases, pharmaceutical formulations, etc., can solve the problem of single cosmetic effect, etc., and achieve the effect of moisturizing effect
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Embodiment 1
[0021] A skin care earthworm active polypeptide extract is carried out according to the following steps:
[0022] 1) Preparation of raw materials: take fresh and live Indian blue earthworms out of the breeding bed, remove sediment and vermicompost, clean them, filter and dry them, and obtain raw materials;
[0023] 2) Preparation of dried earthworms: put the prepared materials obtained in step 1) in purified water at 20°C, soak for 2 hours according to the mass ratio of the prepared materials and purified water at 1:4, and dry them to obtain dried earthworms;
[0024] 3) Prepare a filtrate: mix the dried earthworms obtained in step 2) with xylitol and trehalose according to the mass ratio of 100:5:1.5, put them into a high and low temperature frequency conversion impact box, and set the temperature range to -4 ℃-45℃, the temperature range is set at 8℃, and the time is 2 hours; filter with a 9000 molecular weight ultrafiltration membrane to obtain a filtrate;
[0025] 4) Prepa...
Embodiment 2
[0028] A skin care earthworm active polypeptide extract is carried out according to the following steps:
[0029] 1) Preparation of raw materials: Take the fresh and live Eisenia worms out of the breeding bed, remove the sediment and vermicompost, clean them, filter and dry them, and obtain the raw materials;
[0030] 2) Preparation of dried earthworms: put the prepared materials obtained in step 1) in purified water at 20°C, soak for 1.5 hours according to the mass ratio of the prepared materials and purified water at 1:5, and then dry them to obtain dried earthworms;
[0031] 3) Prepare a filtrate: mix the dried earthworms obtained in step 2) with xylitol and trehalose according to the mass ratio of 100:5.1:2.2, put them into a high and low temperature frequency conversion impact box, and set the temperature range to -4 ℃-45℃, the temperature range is set at 6℃, and the time is 4 hours; filter with an 8000 molecular weight ultrafiltration membrane to obtain a filtrate;
[0...
Embodiment 3
[0035] A skin care earthworm active polypeptide extract is carried out according to the following steps:
[0036] 1) Preparation of raw materials: Take out the fresh and live Eisenia and European night worms from the breeding bed, remove the sediment and vermicompost, clean them, filter and dry them, and prepare the raw materials;
[0037] 2) Preparation of dried earthworms: put the prepared materials obtained in step 1) into purified water at 20°C, soak for 1.5 hours according to the mass ratio of the prepared materials and purified water at 1:4.5, and dry them to obtain dried earthworms;
[0038] 3) Prepare a filtrate: mix the dried earthworms obtained in step 2) with xylitol and trehalose according to the mass ratio of 100:6.3:1.8, put them into a high and low temperature frequency conversion impact box, and set the temperature range to -4 ℃-45℃, the temperature range is set at 10℃, and the time is 2 hours; filter with a 10000 molecular weight ultrafiltration membrane to ob...
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