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Application of a non-destructive sampling method for Chinese mitten crab

A Chinese mitten crab, non-destructive sampling technology, applied in the application, biochemical equipment and methods, climate change adaptation and other directions, can solve the problems of decreased vitality, infection and death, disability of sampling target individuals, etc., to achieve easy collection, ensure egg holding Simple and feasible effect of hatching and sampling methods

Active Publication Date: 2021-06-01
FRESHWATER FISHERIES RES CENT OF CHINESE ACAD OF FISHERY SCI
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Problems solved by technology

SPF seedlings require that the parents do not carry specific pathogens, and the parents must first be tested for specific pathogens. When live sampling is performed on the economical crustacean breeding parents for molecular testing, because the crustaceans are covered with a hard shell, body surface sampling cannot be performed. Therefore, the method of taking muscle by amputating limbs is usually used. This method causes the disability of the sampling target individual, the decline in vitality, and even some individuals die of infection. Therefore, this method is not suitable for the pathogens of SPF seedlings of some shrimp and crab species that require long-term egg incubation. detection
And if you want to test other tissues, such as hepatopancreas, sperm ovary, etc., you need to kill the test parent, which is even more unfeasible.

Method used

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Embodiment 1

[0024] 1. On December 1, 2016, at the Sheyang Chengxin Ecological Nursery Farm in Jiangsu Province, there were 600 female crabs with an average size of 3.2 taels and 300 male crabs with an average size of 4.5 taels in a pond of 2 mu. They were injected with seawater to stimulate mating ,On December 20, 2016, the seawater was drained and the catch was started, and a total of 497 oviposit crabs were obtained;

[0025] 2. The obtained 497 egg-bearing crabs were fastened to the base of the large claws of the Chinese mitten crab with a tightenable plastic ring with a number number, and each egg-bearing crab was numbered; it was temporarily raised in a cement pool paved with mud at the bottom ;

[0026] 3. Live non-destructive sampling of ovipositor crabs: For the fertilized eggs attached to the external appendages of the abdomen of the mother of Chinese mitten crabs, use sterilized clean forceps to pick up 2mm 3 The fertilized eggs (about 50 eggs) were divided into 2 numbered Eppe...

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Abstract

The invention discloses a non-destructive sampling method and application of living body of Chinese mitten crab. By sampling the fertilized eggs of the ovipositous parents of the Chinese mitten crab, the DNA and RNA thereof are extracted to detect related pathogens, and the parents not containing the detection target pathogen are tested. SPF megalopa of Eriocheir sinensis was obtained by isolation cultivation and artificial propagation. The method has the following advantages: the sampling does not damage the mother body, ensures the normal egg hatching of the parent crab, and the obtained megalopa of the Chinese mitten crab does not carry specific pathogens. The present invention solves the problem of non-destructive live sampling in SPF seedling cultivation of Chinese mitten crab, provides a simple and feasible way for SPF seedling cultivation of Chinese mitten crab, and is expected to cultivate SPF healthy seedlings and improve the economic benefits of Chinese mitten crab cultivation .

Description

technical field [0001] The invention relates to the field of SPF seedling cultivation of economic crustaceans and other application fields requiring detection of specific pathogens, in particular to a non-destructive sampling method for shrimps and crabs with ovipositing habits and the application of SPF seedling cultivation. Background technique [0002] Chinese mitten crab (Eriocheir sinensis), also known as river crab, is an important economic aquatic animal in my country. It is widely distributed in rivers and lakes in the north and south of China. The known viruses of Chinese mitten crab include: reovirus (EsRV816, 905 and 2012), bunya virus (EsBV), and white spot syndrome virus (WSSV). These viruses all have the characteristics of vertical transmission. The known Eriocheir sinensis virus can make Eriocheir sinensis pathogenic and even fatal under certain conditions. [0003] Due to the prevalence of White Spot Syndrome Virus (WSSV), Specific pathogen free (SPF, Speci...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01K61/59A01K61/90C12N15/10C12Q1/686C12Q1/6844
CPCA01K61/59A01K61/90C12N15/1013C12Q1/6844C12Q1/686C12Q2531/119Y02A40/81
Inventor 沈怀舜张晓戴天豪
Owner FRESHWATER FISHERIES RES CENT OF CHINESE ACAD OF FISHERY SCI
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