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A method for identifying heterosis patterns of crop varieties based on molecular marker technology

A hybrid vigor and molecular marker technology, applied in the fields of hybridization, genomics, biological systems, etc., can solve the problems of inability to carry out experimental identification, increase restraint conditions, reduce the possibility of accurate pedigree information, etc., to achieve crop universality, saving The effect of time and human and financial resources, good social benefits and economic value

Active Publication Date: 2022-02-08
BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES
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AI Technical Summary

Problems solved by technology

Under normal circumstances, the source of the variety is provided in the variety registration announcement, but this information may be encrypted for the purpose of protecting commercial secrets, which greatly reduces the possibility of obtaining accurate pedigree information
[0009] To sum up, the traditional method needs to know the pedigree information of the hybrid and the information of the inbred line group corresponding to the pedigree, which adds constraints to the identification process. If the above conditions are unknown, the experimental identification cannot be carried out.
Therefore, the existing methods are restricted by many factors and thus have certain limitations.

Method used

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  • A method for identifying heterosis patterns of crop varieties based on molecular marker technology
  • A method for identifying heterosis patterns of crop varieties based on molecular marker technology
  • A method for identifying heterosis patterns of crop varieties based on molecular marker technology

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Embodiment 1

[0129] This example illustrates the specific implementation of maize heterosis pattern identification based on SSR molecular markers.

[0130] Among the selected experimental objects, the control samples were the molecular marker data of 40 SSRs from 135 domestic maize inbred lines (FengGe Wang et al, Development and characterization of a core set of SSRmarkers for fingerprinting analysis of Chinese maize varieties, Maydica, 2011, 56-1693. Hereinafter referred to as paper 1), the samples to be tested are the molecular marker data of 40 SSRs of 10 domestically popularized maize hybrids with the same loci in paper 1 (Wang Fengge et al., 328 maize varieties in China ( Combination) SSR marker genetic diversity analysis, Chinese Agricultural Sciences, 2014,47 (5): 856-864. Hereinafter referred to as paper 2), the crop heterosis pattern identification method based on molecular marker technology proposed by the present invention is verified, specifically The implementation process is...

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Abstract

The invention provides a method for identifying the heterosis pattern of crop varieties based on molecular marker technology, comprising the following steps: (1) establishing a fingerprint information database of crop control inbred lines; (2) building a virtual hybrid combination fingerprint database and determining the heterosis pattern Weight library and threshold; (3) Establish the fingerprint library of the hybrids to be tested; (4) Analyze the heterosis pattern of the hybrids to be tested: Calculate the score value of the hybrids in each heterosis pattern, by comparing with the established weight library The comparison with the threshold value determines the heterosis mode of the hybrid to be tested, and the heterosis mode with the highest score is the heterosis mode of the hybrid. The identification method of the invention can quickly and effectively identify the heterosis pattern of corn hybrids, and can be applied to various aspects such as identification of parents, variety identification, variety purity, variety protection, etc., saves time, human and financial resources, and is convenient for seed companies, breeders and Seed management department to supervise and manage, the application prospect is broad.

Description

technical field [0001] The invention relates to the technical field of identification and breeding of crop hybrids, in particular to the technical field of bioinformatics analysis of crop varieties, in particular to a method for identifying heterosis patterns of crop hybrids based on molecular marker technology. Background technique [0002] The most prominent contribution of plant genetics to the development of agricultural production in the 20th century is the development and utilization of heterosis. After the rediscovery of Mendel's genetic law, the concept of hybrids between inbred lines was proposed. Breeders bred a large number of inbred lines and prepared a large number of hybrid combinations, which made the commercial use of corn hybrids develop by leaps and bounds. Heterosis breeding is the mainstream of maize breeding today, and popularizing and planting strong hybrids plays a key role in increasing maize yield. In crop breeding at home and abroad, choosing a rea...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G16B5/00G16B20/20G16B50/00G16B40/00
CPCG16B25/00G16B40/00
Inventor 杨扬王凤格赵久然田红丽王璐
Owner BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES
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