Method for regulating cellular pathway by using plant hormone GA (Gibberellic Acid) and small molecular substance PAC (Paclobutrazol)
A technology of small molecular substances and plant hormones, applied in the field of biological method invention, can solve the problems of immune system damage and incompatibility
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[0037]A method for regulating cell pathways by utilizing the plant hormone GA and the small molecular substance PAC, comprising the following methods:
[0038]
[0039] (1) Carrier design and construction:
[0040] First, using the primers described above, using the Arabidopsis thaliana cDNA and the HEK293T cell cDNA as templates, use pcr technology to amplify each gene fragment, and then use the corresponding primers to perform overlap PCR with two fragments as templates; The Arabidopsis DELLA protein GAI and the GA receptor GID1 were fused with the C-terminus of the key protein LRP6 in the Wnt signaling pathway, and the PCR products such as GAI, GID1, GAI-LRP6C, GID1-LRP6C were recovered by using agarose gel, and used in Dicer XbaI XmaI double-digest linearized pCI 4xmyc, pCI egfp and pCI mcherry vectors, use the Infusion method to connect, transform DH5a, colony PCR verification, pick clones and send them for sequencing, and ensure correct sequencing; use the above opera...
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