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Collagen-fibroin co-assembly sponge material, co-assembly artificial skin and preparation method of sponge material and co-assembly artificial skin

一种人工皮肤、共组装的技术，应用在组织再生、涂层、医药科学等方向，能够解决人工皮肤降解周期难以控制、真皮层多孔结构杂乱、多孔结构不规则等问题，达到提高愈合质量、促进创面愈合、良好生物相容性的效果

Active Publication Date: 2018-08-17
HUBEI CENT MEDICAL TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the mechanical properties of the composite porous scaffold of the dermis are slightly improved, due to the large differences in the structure and performance of collagen and 6-chondroitin sulfate, the artificial skin has shortcomings and defects such as difficult to control the degradation cycle and irregular porous structure.
In the Chinese invention patent "a double-layer collagen dermal scaffold and its preparation method" with the publication number CN 107050521 A, the epidermis is a silica gel membrane, and the dermis is a collagen-chondroitin sulfate composite scaffold, and the porous structure of the dermis is also disordered. insufficient

Method used

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  • Collagen-fibroin co-assembly sponge material, co-assembly artificial skin and preparation method of sponge material and co-assembly artificial skin
  • Collagen-fibroin co-assembly sponge material, co-assembly artificial skin and preparation method of sponge material and co-assembly artificial skin
  • Collagen-fibroin co-assembly sponge material, co-assembly artificial skin and preparation method of sponge material and co-assembly artificial skin

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] (1) Weigh an appropriate amount of atelopeptide type I collagen, and prepare a collagen solution with a concentration of 2.5 μmol / L with 0.5 mol / L acetic acid solution. Weigh an appropriate amount of silkworm silk fibroin freeze-dried powder, dissolve it in a calcium chloride ternary system solution (calcium chloride: ethanol: water molar ratio 1:2:8), and dialyze with pure water in a dialysis bag with a molecular weight cut-off of 3500Da. Prepare a silk fibroin solution with a concentration of 2.5 μmol / L.

[0040] (2) Take 2.5 μmol / L collagen solution and 2.5 μmol / L silk fibroin solution, mix them in equal volumes, stir them mechanically at 200 rpm and 0°C for 8 hours, and then mix them under the conditions of 0°C and 40% humidity Co-assembled for 48 hours (collagen-silk fibroin co-assembled product), the mixed solution after co-assembly was frozen at four temperature points of 0°C, -20°C, -40°C, and -80°C for 12h respectively, and then at -80°C , Dry under 1Pa condit...

Embodiment 2

[0055] (1) Weigh an appropriate amount of atelopeptide type I collagen, and prepare a collagen solution with a concentration of 5.0 μmol / L with 0.5 mol / L acetic acid solution. Weigh an appropriate amount of silkworm silk fibroin freeze-dried powder, dissolve it in a calcium chloride ternary system solution (calcium chloride: ethanol: water molar ratio 1:2:8), and dialyze with pure water in a dialysis bag with a molecular weight cut-off of 3500Da. Prepare a silk fibroin solution with a concentration of 5.0 μmol / L.

[0056] (2) Take 5.0 μmol / L collagen solution and 5.0 μmol / L silk fibroin solution, mix them in equal volumes, mechanically stir for 4 hours under the conditions of rotating speed 150 rpm and temperature 20°C, and then mix them under the conditions of temperature 20°C and humidity 60%. The mixed solution was frozen at four temperature points of 0°C, -20°C, -40°C, and -80°C for 24h, and then dried at -80°C and 1Pa for 60h. The obtained freeze-dried product was cross-...

Embodiment 3

[0059] (1) Weigh an appropriate amount of atelopeptide type I collagen, and prepare a collagen solution with a concentration of 10.0 μmol / L with 0.5 mol / L acetic acid solution. Weigh an appropriate amount of silkworm silk fibroin freeze-dried powder, dissolve it in a calcium chloride ternary system solution (calcium chloride: ethanol: water molar ratio 1:2:8), and dialyze with pure water in a dialysis bag with a molecular weight cut-off of 3500Da. Prepare a silk fibroin solution with a concentration of 10.0 μmol / L.

[0060] (2) Take 10.0 μmol / L collagen solution and 10.0 μmol / L silk fibroin solution, mix them in equal volumes, mechanically stir for 1 hour under the conditions of rotating speed 100 rpm and temperature 37°C, and then mix them under the conditions of temperature 37°C and humidity 80%. The combined solution was assembled at 0°C, -20°C, -40°C, and -80°C for 36 hours, and then dried at -80°C and 1 Pa for 72 hours. The obtained freeze-dried product was cross-linked ...

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PUM

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Abstract

The invention relates to a collagen-fibroin co-assembly sponge material, a co-assembly artificial skin and a preparation method of the sponge material and the co-assembly artificial skin. The collagen-fibroin co-assembly sponge material is provided with an ordered porous space structure and prepared from collagen-fibroin co-assembly products obtained by co-assembling collagen and fibroin. The co-assembly artificial skin comprises a collagen-fibroin co-assembly sponge corium layer and a cuticular layer. The composition and the structure of the corium layer of the co-assembly artificial skin arecloser to those of a real extracellular matrix, the ordered novel structure can induce ordered arrangement and regeneration of cells, wound healing is promoted, scar formation is decreased, and the co-assembly artificial skin has good biocompatibility.

Description

technical field [0001] The invention relates to a collagen-silk fibroin co-assembled sponge material, a co-assembled artificial skin and a preparation method thereof, belonging to the field of biomedical materials. Background technique [0002] The skin is one of the largest and most important organs in the human body, performing functions such as respiration, maintaining fluid loss and biological barrier. Large-area skin defects caused by severe burns, deep burns, severe trauma, infection, ulcers, etc. are a major clinical problem. At present, the commonly used methods include autologous skin transplantation, allogeneic skin transplantation, and xenogeneic skin transplantation, but there is often a supply Insufficiencies and defects such as insufficiency, immune rejection and easy transmission of diseases. Applying the principles and technologies of biomaterials and tissue engineering, an artificial skin with a structure and function similar to normal human skin has been d...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/26A61L27/60A61L27/56A61L27/58
CPCA61L27/26A61L27/56A61L27/58A61L27/60A61L2430/34C08L89/00A61L27/24A61L27/34A61L27/3604A61L2430/40
Inventor 王江林叶红川刘泽慧杜莹莹
Owner HUBEI CENT MEDICAL TECH CO LTD
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