Test strip capable of detecting organophosphorus and carbamate pesticide residues in edible agricultural products through one-step method

Abstract

The invention provides a test strip capable of detecting organophosphorus and carbamate pesticide residues in edible agricultural products. The test strip is an agricultural residue detection test strip composed of a substrate sheet with the diameter of 11 mm to 20 mm, cholinesterase with the viability unit of 40 to 75 U, and liquid indophenol acetate with absorbance of 0.2 at 490 nm. In detection, a sample is pretreated, a drop of the pretreated solution of the sample is added into the test strip and undergoes a reaction at 37 DEG C for 6 min, the color development result is observed, and ifthe color is blue, there are not pesticide residues, if the color is white, there are organophosphorus and carbamate pesticide residues. The pesticide residue card manufacturing process and the detection process do not need expensive instruments and reagents, are easy to operate and have high sensitivity.

Description

technical field [0001] The invention relates to the technical field of bioengineering detection, adopts a one-step method to detect organic phosphorus and carbamate pesticide residues in edible agricultural products, and specifically relates to a method for realizing the coexistence of enzymes and substrates on a substrate sheet without catalytic reaction Pesticide residue detection test paper. Background technique [0002] In agricultural production, chemical pesticides will be used for a long period of time. If pesticides are not applied according to the prescribed dosage, frequency, method or safety interval, or pesticides that should not be used are used, it will cause pesticides to exceed the standard or Pesticide poisoning. [0003] Among the pesticides in our country, 70% are organophosphorus pesticides, and in the production and use of organophosphorus pesticides in our country, 70% are highly toxic and highly toxic, and most of them are prohibited from being used o...

AI Technical Summary

Problems solved by technology

[0005] At present, there are mainly three types of rapid detection cards for pesticide residues. One is the pesticide residue card that needs to be folded in half (such as the rapid test card method in GB / T5009.199-2003). This card has cumbersome operations and insufficient manual folding. The problem of long detection time and poor anti-interference ability; the second is a one-step pesticide residue card, for example, in the patent publication number CN104655621A, the chromogenic pad and the enzyme pad are fixed on a bottom plate in turn, and the chromogenic pad is fixed on the reaction process. The chromogenic substance fails to fully catalyze the reaction with the cholinesterase on the enzyme pad

However, the current detection method still has the problems of long detection time, cumbersome operation, and high sensitivity. Therefore, the development of a detection test paper that is more suitable for the rapid screening of organophosphorus and carbamate pesticide residues at the grassroots level is important to protect the health of the people. health matters

Images