Amphibian isolated heart perfusion device

Abstract

The invention relates to an amphibian isolated heart perfusion device. The device comprises an arterial cannula, a venous cannula, a drip recording electrode and an electrocardio lead electrode, wherein the arterial cannula is formed by combining an arterial cannula trunk, an aorta inserting end and a liquid discharging pipe through a three-way valve; the venous cannula is formed by combining a liquid storage tank, a postcaval vein inserting end and a liquid adding pipe through a three-way valve, and parts of the arterial cannula and the venous cannula are inserted and combined into one. The drip recording electrode comprises a drip recording electrode head, a drip recording electrode rod and a signal input end; the electrocardio lead electrode comprises an electrocardio lead electrode head, an electrocardio lead electrode rod, a signal input end, a heart outer wall liquid adding pipe and a three-way valve. The multifunctional amphibian isolated heart perfusion device is suitable for one person to operate, facilitates perfusion liquid replacement, and is suitable for synchronously and dynamically detecting dynamical changes of the cardiac output, myocardial contraction tension, electrocardiogram and various cardiac function indexes.

Description

technical field [0001] The invention relates to an experimental device for scientific research and experimental teaching related to life sciences, in particular to an amphibian isolated heart perfusion device. Background technique [0002] Using the isolated heart perfusion system can visually observe the activity of the heart, study the systolic function of the heart, the change of heart rate and rhythm, and the influence of various body fluid factors on the heart function. It is one of the important means to study the heart function and is widely used in medicine. , biology, pharmacy and other teaching and research. The traditional double-tube method (Yagi-Hartung method) cardiac perfusion device using the isolated heart of amphibians such as toads or frogs can visually observe the overall cardiac contraction, does not require constant temperature and oxygen supply equipment, and cardiac activity is not regulated by neurohumoral Etc. The one-way circulating flow of perfu...

AI Technical Summary

Problems solved by technology

However, due to the structural characteristics of the perfusion device itself, the traditional double-tube method makes the preparation of perfusion specimens more complicated and difficult for one person to operate; it needs to use a straw to add or draw liquid, which is inconvenient for flushing or changing liquid; sequentially applying different perfusate ( When using a straw to draw liquid, it is easy to touch the inner wall of the device, which will affect the measurement of cardiac contraction tension and other experimental results; only cardiac output can be detected, and the detection index is single, which is not conducive to multi-angle analysis of cardiac physiological functions

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