Method for identifying western Henan black pigs by using 12S rRNA gene mutation sites

Abstract

The invention relates to the field of animal genetics, in particular to a method for identifying western Henan black pigs by using 12S rRNA gene mutation sites. The method comprises the steps of downloading the 12S rRNA gene in a reference sequence in NCBI; respectively designing a PCR primer at the upstream part and the downstream part of the 314 site basic group of the 12S rRNA gene; extractingDNA of a sample to be tested; performing PCR amplification by using the DNA of the sample to be tested as a template and the PCR primers in the step (2) as primers; performing electrophoresis gel running (agarose gel electrophoresis) identification on the PCR product, and recovering a target product; comparing the target product subjected to sequencing with the 12S rRNA gene of the western Henan black pigs to complete the identification of the western Henan black pigs. The method solves the problem of distinguishing among the black pigs in the same region, and also solves the problems that theexisting pork market is disordered, the defective pork is used as high-quality pork, and an effective detection method does not exist.

Description

technical field [0001] The invention relates to the field of animal genetics, in particular to a method for identifying Henan black pigs by utilizing mutation sites of 12S rRNA genes. Background technique [0002] Western Henan black pigs are mainly distributed around Lushi County, Sanmenxia District, Henan Province. After long-term natural selection, the local mountainous people have artificially selected and bred local pig breeds according to their own feeding habits and living and consumption habits, and adapted to the local breeding environment conditions. It is resistant to rough feeding, adaptable, strong disease resistance, and good meat quality. It has a greater market influence in the western Henan region, and is habitually called the Western Henan black pig by industry insiders. [0003] Mitochondrial genome is a relatively conserved circular DNA sequence in the animal genome. It has the characteristics of strong conservation and fast evolution, and is an important...

AI Technical Summary

Problems solved by technology

[0005] The present invention proposes a method for identifying black pigs in Western Henan by using the mitochondrial 12S ribosomal RNA (12S rRNA) gene, which solves the problem of confusion in the existing pork market and the use of substandard pork as high-quality pork, but there is no effective detection method. The inventors are studying During the process, it was found that the 314th base of the 12S rRNA gene of Yuxi black pigs was compared with the pig 12S rRNA reference sequence SEQ ID 1 on NCBI. In the 12S rRNA sequence SEQ ID 2 of Yuxi black pigs, the 314th base was T is mutated to C, and this application uses this finding to identify the breed of pigs

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