Tissue culture and rapid propagation method of lemon

Abstract

The invention discloses a tissue culture and rapid propagation method of lemon. The tissue culture and rapid propagation method comprises: (1) culture starting: cutting lemon branch with bud stem segment as explant, sterilizing, and inoculating into a starting culture medium, and culturing for 4-5 weeks until axillary buds are sprouted on the explant and grow to achieve a certain length; (2) proliferation culture: cutting the axillary buds obtained in the step (1), inoculating into a proliferation culture medium, and culturing for 4-6 weeks until clustered buds are formed on the base portionsof the axillary buds and grow to achieve a certain length; and (3) rooting culture: cutting the clustered buds obtained in the step (2) into single buds, inoculating the single buds into a rooting culture medium, and culturing for 4-5 weeks until roots are generated and grow to achieve a certain length so as to obtain the lemon tissue culture seedlings. According to the present invention, the culture time of the seedling obtained through the method is short; compared to traditional grafting and cutting, the tissue culture and rapid propagation method of the present invention can significantlyincrease the proliferation coefficient of lemon, increase the survival rate, ensure the genetic stability, and conveniently perform the unified transplanting and planting management at the later period so as to achieve the rapid propagation of lemon.

Description

technical field The invention relates to a method for tissue culture and rapid propagation of plants, in particular to a method for tissue culture and rapid propagation of lemons. Background technique Lemon, Rutaceae citrus plant, lemon is also called lemon fruit, foreign lemon, mother fruit and so on. Branches with few thorns or nearly no thorns, young leaves and flower buds dark purple, thick papery leaves, ovate or ellipse. Single flowers axillary or few flowers clustered. Fruit oval or ovate, with thick peel, usually rough, sour to very sour juice, small, ovate, apex; seed coat smooth, cotyledons milky white, usually single or with multiple embryos. The flowering period is from April to May, and the fruiting period is from September to November. Lemon is an important natural spice, food and industrial raw material, and it is used in food, medicine, cosmetics, daily chemical industry and other industries. The existing lemon cultivation method is generally cutting or g...

AI Technical Summary

Problems solved by technology

The existing lemon cultivation methods are generally cutting or grafting, which has a low reproductive coefficient, slow growth, and low survival rate. In addition, it is not conducive to the maintenance of its genetic stability.

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