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Method for rapid propagation of pelargonium by means of tissue culture

A tissue culture and geranium technology, applied in the field of plant tissue culture, can solve the problems such as the limitation of the number and speed of reproduction, and achieve the effect of maintaining the advantage of the strain, increasing the number and speed, and reducing the size

Inactive Publication Date: 2018-05-22
SHANDONG CROP GERMPLASM CENT
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In actual production, geraniums are mostly propagated by cuttings, but cuttings require a certain length of branches as cuttings, and the number and speed of propagation are limited.

Method used

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  • Method for rapid propagation of pelargonium by means of tissue culture
  • Method for rapid propagation of pelargonium by means of tissue culture
  • Method for rapid propagation of pelargonium by means of tissue culture

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] A tissue culture rapid propagation method of Pelargonium, the steps are:

[0037] (1) Selection and disinfection of explants

[0038] Select young geranium leaves with a clean surface as explants, first soak them in 75% ethanol for 1 min, then sterilize them with sodium hypochlorite solution containing 5% available chlorine on a clean bench for 5 min, and finally rinse them with sterile water for 5 min. times, 1 min each time, to obtain sterile explants;

[0039] callus induction

[0040] Cut the sterile explant in step (1) into 0.5*0.5cm pieces, remove the leaf edge and veins, first cut a wound on the back of the leaf (the length of the wound on the back of the leaf is 0.4-0.55cm, and the wound should avoid the veins position, do not cut through, and keep the upper surface of the leaf intact), then inoculate the back of the leaf on the callus induction medium downward, and culture in the dark at 25°C for one week, then follow the cycle of "light culture 16h-dark c...

Embodiment 2

[0049] A tissue culture rapid propagation method of geranium, the difference from Example 1 lies in: step (2) callus induction: two wounds crossing 60 degrees are cut at the center position of the back of the leaf.

Embodiment 3

[0051] A tissue culture rapid propagation method of geranium, which is different from Example 1 in that: step (3) induces the differentiation medium to obtain plants, and after the young shoots are differentiated, continue to culture for 3 to 4 weeks: the culture process is specifically: According to the cycle of "light culture 12h-dark culture 12h", continue to culture at 18-25℃ for another 2-3 weeks, and then follow the cycle of "light culture 16h-dark culture 8h", continue to culture at 22-25℃ 1 to 2 weeks.

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Abstract

The invention provides a method for rapid propagation of pelargonium by means of tissue culture. The method comprises the steps of selecting tender leaves, having a good growing status, of the pelargonium as explants, disinfecting the explants, then cutting off the leaf margins and veins of the explants, and inoculating into a callus induction medium for inducing calluses; transferring the inducedcalluses into a differentiation medium for inducing the production of buds; inoculating the induced effective plants into a rooting medium for carrying out rooting culture; the rooting of the plantsis completed and complete plants grow out, enabling the plants to be subjected to hardening and transplanting. According to the method, pelargonium seedlings are obtained by carrying out tissue culture on the tender leaves of the pelargonium; the method is easy in obtaining of materials, convenient in disinfection and high in callus induction success rate; after the calluses are differentiated, the seedling yield is high; although the test period of the method is slightly longer than that of the direct cuttage, the plant propagation efficiency of the method is much higher than that of cuttage;furthermore, the method can better maintain the strain advantages of the pelargonium and can rapidly reproduce a large number of pelargonium seedlings suitable for transplanting.

Description

technical field [0001] The invention relates to the technical field of plant tissue culture, in particular to a tissue culture rapid propagation method of geranium. Background technique [0002] Geranium ( Pelargonium ) is the Bovine family ( Geraniaceae ) Pelargonium ( Pelargonium ) perennial herbaceous flowers, also known as hydrangea, wax red, stone wax red, geranium succulent stems, single leaves alternate, umbels, rich and bright colors, is a common flower in various places. At present, there are many potted plants in various parts of our country, and the cultivation is very common. They are potted flowers for viewing leaves and flowers, and are also important flower bed materials. [0003] Geranium propagation methods include sowing and cutting propagation, but the anthers of geranium degenerate, the pistils are mostly sterile, and occasionally they can bear fruit, and the capsule has only one seed. In actual production, geraniums are mostly propagated by cuttings...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 丁汉凤李娜娜蒲艳艳宫永超郭秀秀
Owner SHANDONG CROP GERMPLASM CENT
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