Skin-whitening anti-oxidation plant composition, plant composition fermenting liquid as well as preparation method and application thereof
A technology of composition and fermented liquid, which is applied in the field of daily chemicals, can solve the problems of high irritation, single effect, instability, etc., and achieve the effect of improving whitening, high safety, and mild reaction conditions
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Embodiment 1
[0057] This embodiment prepares the fermented liquid of plant composition through the following steps
[0058] (1) Weigh 5.2g of Rhizoma Chuanxiong, 3.9g of Radix Paeoniae Rubra, 2.6g of Radix Radix Radix and 1.3g of mulberry leaves in proportion, pulverize, pass through a 50-mesh sieve, and mix 10 6 -10 8 The cfu / mL Lactobacillus gasseri bacterial solution was inoculated into the plant composition and sterile water, the ratio of the plant composition, water and bacterial solution was 13g:200g:20mL, in a shaker at 37°C with a rotation speed of 100r / min Carry out fermentation 48h, obtain fermentation product;
[0059] (2) The fermentation product was centrifuged at a rotational speed of 12000r / min for 30min, wherein the centrifugal radius was 9cm, the supernatant was collected, passed through a 0.22μm filter membrane, sterilized by filtration, and the filtrate was collected to obtain a plant composition fermentation broth.
Embodiment 2
[0061] This embodiment prepares the fermented liquid of plant composition through the following steps
[0062] (1) Weigh 4.55g of Rhizoma Chuanxiong, 3.25g of Radix Paeoniae Rubra, 2.6g of Radix Radix Radix and 2.6g of mulberry leaf in proportion, pulverize, after passing through a 50-mesh sieve, put 10 6 -10 8 The cfu / mL Lactobacillus gasseri bacterial solution was inoculated into the plant composition and sterile water, the ratio of the plant composition, water and bacterial solution was 13g:200g:20mL, in a shaker at 37°C with a rotation speed of 100r / min Carry out fermentation 48h, obtain fermentation product;
[0063] (2) The fermentation product was centrifuged at a rotational speed of 12000r / min for 30min, wherein the centrifugal radius was 9cm, the supernatant was collected, passed through a 0.22μm filter membrane, sterilized by filtration, and the filtrate was collected to obtain a plant composition fermentation broth.
Embodiment 3
[0065] This embodiment prepares the fermented liquid of plant composition through the following steps
[0066] (1) Weigh 3.9g of Rhizoma Chuanxiong, 3.25g of Radix Paeoniae Rubra, 3.25g of Radix Paeoniae Alba and 2.6g of mulberry leaves in proportion, grind them, and after passing through a 50-mesh sieve, put 10 6 -10 8 The cfu / mL Lactobacillus gasseri bacterial solution was inoculated into the plant composition and sterile water, the ratio of the plant composition, water and bacterial solution was 13g:200g:20mL, in a shaker at 37°C with a rotation speed of 100r / min Carry out fermentation 48h, obtain fermentation product;
[0067] (2) Centrifuge the fermentation product for 30min at a rotating speed of 12000r / min, wherein the centrifugal radius is 9
[0068] cm, the supernatant was collected, passed through a 0.22 μm membrane filter, sterilized by filtration, and the filtrate was collected to obtain a plant composition fermentation broth.
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