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Artificial dendrobium huoshanense breeding method

A kind of artificial and rice dendrobium technology, applied in the field of breeding, can solve the problems of difficult transplanting, low survival rate, slow rooting of test tube seedlings, etc., and achieve the effect of rich nutritional value, high reproduction coefficient, and short growth cycle

Pending Publication Date: 2018-05-15
徐小毛
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But test-tube seedlings take root slowly, transplanting is difficult, and the survival rate after transplanting is relatively low,

Method used

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  • Artificial dendrobium huoshanense breeding method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036]The artificial breeding method of rice dendrobium comprises the following steps:

[0037] S1 Sowing: Disinfect the fruit of Dendrobium officinalis with 1% sodium hypochlorite aqueous solution for 60 minutes, rinse with sterile distilled water for 5 minutes, cut the fruit, spread the seeds evenly on the surface of the solid sowing medium, and the sowing amount of the seeds is 7 / mL medium, wherein the composition of the solid seeding medium is: 30g / L sucrose, 7g / L agar, 1650mg / L NH 4 NO 3 , 1900mg / L KNO 3 , 370mg / L MgSO 4 ·7H 2 O, 4400mg / L CaCl 2 2H 2 O, 170mg / L KH 2 PO 4 , the balance is water;

[0038] S2 seeds germinated to form seedlings: cultivated for 6 months after sowing based on the conditions of temperature 25°C, air humidity 80%, light intensity 1600lx, and light time 12 hours / day, and the seeds germinated to form seedlings;

[0039] S3 explants induce protocorms: select seedling stems with a length of 1.2 cm as explants, and inoculate the stems in sol...

Embodiment 2

[0044] The artificial breeding method of rice dendrobium comprises the following steps:

[0045] S1 Sowing: Disinfect the fruit of Dendrobium officinalis with 1% sodium hypochlorite aqueous solution for 60 minutes, rinse with sterile distilled water for 5 minutes, cut the fruit, spread the seeds evenly on the surface of the solid sowing medium, and the sowing amount of the seeds is 7 / mL medium, wherein the composition of the solid seeding medium is: 30g / L sucrose, 7g / L agar, 1650mg / L NH 4 NO 3 , 1900mg / L KNO 3 , 370mg / L MgSO 4 ·7H 2 O, 4400mg / L CaCl 2 2H 2 O, 170mg / L KH 2 PO 4 , the balance is water;

[0046] S2 seeds germinated to form seedlings: cultivated for 6 months after sowing based on the conditions of temperature 25°C, air humidity 80%, light intensity 1600lx, and light time 12 hours / day, and the seeds germinated to form seedlings;

[0047] S3 explants induce protocorms: select seedling stems with a length of 1.2 cm as explants, and inoculate the stems in so...

Embodiment 3

[0052] The artificial breeding method of rice dendrobium comprises the following steps:

[0053] S1 Sowing: Disinfect the fruit of Dendrobium officinalis with 1% sodium hypochlorite aqueous solution for 60 minutes, rinse with sterile distilled water for 5 minutes, cut the fruit, spread the seeds evenly on the surface of the solid sowing medium, and the sowing amount of the seeds is 7 / mL medium, wherein the composition of the solid seeding medium is: 30g / L sucrose, 7g / L agar, 1650mg / L NH 4 NO 3 , 1900mg / L KNO 3 , 370mg / L MgSO 4 ·7H 2 O, 4400mg / L CaCl 2 2H 2 O, 170mg / L KH 2 PO 4 , the balance is water;

[0054] S2 seeds germinated to form seedlings: cultivated for 6 months after sowing based on the conditions of temperature 25°C, air humidity 80%, light intensity 1600lx, and light time 12 hours / day, and the seeds germinated to form seedlings;

[0055] S3 explants induce protocorms: select seedling stems with a length of 1.2 cm as explants, and inoculate the stems in so...

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Abstract

The invention discloses an artificial dendrobium huoshanense breeding method. The method includes steps: S1, seeding; S2, seed germination and seedling growth; S3, explant induction into protocorms; S4, subculture; S5, protocorm multiplication. The artificial dendrobium huoshanense breeding method has advantages that by means of tissue culture, short growth period and high propagation coefficientare realized, and the current condition of high dendrobium huoshanense demand is met; in addition, obtained dendrobium huoshanense is high in nutritive value and has efficacies of throat clearing andnourishing, eyesight improving, summer heat relieving, Qi tonifying, stomach nourishing and heat clearing.

Description

technical field [0001] The invention relates to the technical field of breeding, in particular to a method for artificial breeding of Dendrobium officinalis. Background technique [0002] Dendrobium huoshanense, commonly known as rice dendrobium, was first seen in Zhao Xuemin's "Supplements to Materia Medica Steel Eyes" in the Qing Dynasty, with a history of more than 200 years. The book records: "Huo Dendrobium came out of Huoshan, Jianghuai. It looks like a small hairpin, yellow in color and not straight. Sweet, slightly salty, the shape is reduced to true". The book quoted Nian Xiyao's experience as saying: "Sweet Dendrobium is used for Changsheng Dan, that is, Dendrobium Huoshan." Cistanche, Shenshan Ganoderma lucidum, sea pearl, and Cordyceps sinensis are listed as the "Nine Immortal Grasses" in China, and Huoshan Dendrobium ranks first. [0003] As a rare and valuable Chinese medicinal material, Dendrobium has long been well-known at home and abroad, and the current...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 徐小毛
Owner 徐小毛
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