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Method for extracting protein oral liquid by using marine product waste

A technology of protein oral liquid and waste, which is applied in the field of preparation of protein nutritional products, can solve the problems of high histamine content, achieve the effects of protecting the environment, avoiding rot, and reasonable technology

Inactive Publication Date: 2018-05-08
荣成市飞创科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In order to overcome the deficiencies in the prior art, the purpose of the present invention is to provide a method for extracting protein oral liquid from marine waste with reasonable technology and reliable technology, and to overcome the problem of excessive histamine content

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] In this embodiment, a method for extracting protein oral liquid from marine wastes comprises the following steps:

[0024] (I) Pretreatment of animal marine waste: remove shellfish shells and obviously rotten parts of marine waste, and crush the marine waste into 5-10 mm sized particles, and mix with 1 times the weight of water ;

[0025] (II) Add trypsin to 55 parts by weight of the marine waste in step I to 4500U / mL, and enzymolyze it at 42 degrees Celsius for 16 hours, and heat it at 85 degrees Celsius for 15 minutes to inactivate;

[0026] (III) Add alkaline protease to 2400U / mL to 120 parts by weight of the marine waste in step I, and perform enzymatic hydrolysis at 42 degrees Celsius for 16 hours, and heat at 85 degrees Celsius for 15 minutes to inactivate;

[0027] (IV) Mix the enzymolysis mixture from steps II and III, and mix according to 2.0×10 6 The amount of CFU / mL was inoculated with abnormal Wickham yeast with deposit number CGMCC2.341, fermented at 42 ...

Embodiment 2

[0030] In this embodiment, a method for extracting protein oral liquid from marine wastes comprises the following steps:

[0031] (I) Pretreatment of animal marine waste: remove shellfish shells and obviously rotten parts of marine waste, and crush the marine waste into 5-10 mm sized particles, and mix with 1.5 times the weight of water ;

[0032] (II) Add trypsin to 3000 U / mL to 50 parts by weight of the marine waste in step I, and enzymolyze it at 45 degrees Celsius for 18 hours, and heat it at 85 degrees Celsius for 30 minutes to inactivate;

[0033] (III) adding alkaline protease to 100 parts by weight of the marine waste of step I to 4000 U / mL, enzymatically hydrolyzing at 40 degrees Celsius for 12 hours, and heating at 95 degrees Celsius for 15 minutes to inactivate;

[0034] (IV) Mix the enzymolysis mixture from steps II and III, and 6 The amount of CFU / mL was inoculated with abnormal Wickham yeast with deposit number CGMCC2.341, fermented at 40 degrees Celsius for 1...

Embodiment 3

[0037] In this embodiment, a method for extracting protein oral liquid from marine wastes comprises the following steps:

[0038] (I) Pretreatment of animal marine waste: remove shellfish shells and obviously rotten parts of marine waste, and crush the marine waste into 5-10 mm sized particles, and mix with 1.2 times the weight of water ;

[0039] (II) Add trypsin to 6000 U / mL to 70 parts by weight of the marine waste in step I, and enzymolyze it at 40 degrees Celsius for 12 hours, and heat it at 95 degrees Celsius for 15 minutes to inactivate;

[0040] (III) adding alkaline protease to 1400 U / mL to 110 parts by weight of the marine waste of step I, and enzymatically hydrolyzing at 45 degrees Celsius for 18 hours, and heating at 95 degrees Celsius for 30 minutes to inactivate;

[0041] (IV) Mix the enzymolysis mixture from steps II and III, and mix according to 3.0×10 6 The amount of CFU / mL was inoculated with abnormal Wickham yeast with deposit number CGMCC2.341, fermented...

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Abstract

The invention discloses a method for extracting protein oral liquid by using marine product waste. The method comprises the following steps of (I) pretreating the marine product waste; (II) adding trypsin to the marine product waste, and performing enzymolysis; (III) adding alkali protease to the marine product waste, and performing enzymolysis; (IV) mixing an enzymolysis mixture obtained in stepII with an enzymolysis mixture obtained in step III, and performing inoculating with abnormal Kodomaea ohmeri yeast for fermentation; and (V) filtering a fermentation mixture obtained in step IV to obtain products. In the step IV, the abnormal Kodomaea ohmeri yeast (Kodomaea ohmeri) is preserved in the China General Microbiological Culture Collection Center with the preservation number of CGMCC2.341. The extract prepared by the method is rich in nutrient substances of polypeptide, free amino acids, soluble protein and the like, and has low histamine content.

Description

technical field [0001] The invention relates to the preparation of protein nutritional products, in particular to a method for extracting protein oral liquid from marine waste. Background technique [0002] Coastal fishery cities often produce a large amount of seafood waste in the process of fishery production, which not only wastes resources but also pollutes the environment. If the targeted development of seafood waste can be designed, it will greatly save natural resources and bring good economic benefits. [0003] In the process of economically exploiting seafood waste, one of the difficulties is that the freshness of seafood waste is often difficult to guarantee. Compared with normal seafood raw materials, seafood waste needs to be collected from different production facilities and different production steps, aggregated and used for subsequent processing. During this process, it may have a slight autolysis phenomenon, and lead to an increase in histamine content, res...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23J1/00A23J1/04
CPCA23J1/002A23J1/04A23V2002/00A23V2250/76A23V2300/14A23V2300/38A23V2250/2042
Inventor 张晓燕
Owner 荣成市飞创科技有限公司
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