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Plasmid containing functional gene capable of degrading 4-aminoquinoline

A functional gene, aminoquinoline technology, applied in the field of microbiology and molecular biology, to achieve the effect of improving the biodegradation effect

Active Publication Date: 2018-05-04
BEIJING NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is a lack of research reports on quinoline degradation plasmids at home and abroad.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0022] Below in conjunction with example further illustrate the present invention.

[0023] (1) Add 400mL of basal medium, 1.0mL of trace metal liquid, and 0.1mL of vitamin C solution with a mass concentration of 10mg / L into a 500mL Erlenmeyer flask, shake well and use it as a liquid medium for later use; the basal medium The composition is: NH 4 NO 3 :1.7g·L -1 , KH 2 PO 4 :1.0g·L -1 , K .2 HPO 4 :1.0g·L -1 , MgCl 2 :0.30g·L -1 , CaCl 2 2H 2 O:0.15g·L -1 ; The composition of the trace metal liquid is: CoCl 2 ·6H 2 O:55mg·L -1 , CuCl 2 :0.35mg·L -1 , H 3 BO 3 :11.0mg·L -1 , MnCl 2 4H 2 O:45mg·L -1 , Na 2 MoO 4 2H 2 O:5.5mg·L -1 , NiCl 2 2H 2 O:4.5mg·L -1 , ZnCl 2 :4.5mg·L -1 ;

[0024] (2) Add 4.0mL of 4-aminoquinoline acetone solution with a mass concentration of 0.5g / L to a 100mL conical flask in a clean bench, place the conical flask open for 2 days to remove acetone;

[0025] (3) add 40mL liquid culture medium prepared by step (1) in the E...

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PUM

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Abstract

The invention provides plasmid containing functional gene capable of degrading 4-aminoquinoline. The plasmid containing the functional gene capable of degrading the 4-aminoquinoline is obtained by extracting and purifying bacterial solution of strain Microbacterium sp.M.CWS5. The strain Microbacterium sp.M.CWS5 is collected in China General Microbiological Culture Collection Center on 30th October, 2017 with the strain collection number of CGMCC No. 14840. The plasmid containing the functional gene capable of degrading the 4-aminoquinoline has the beneficial effects that the strain with a degrading function can be constructed for the 4-aminoquinoline, the biological degradation effect of polycyclic aromatic pollutants adopting the 4-aminoquinoline as a representative can be improved, and the plasmid has application potential for soil and water bodies polluted by the polycyclic-aromatic 4-aminoquinoline.

Description

technical field [0001] The invention belongs to the technical field of microbiology and molecular biology, in particular to a plasmid containing a functional gene capable of degrading 4-aminoquinoline. Background technique [0002] Quinoline is a typical nitrogen-containing heterocyclic aromatic hydrocarbon compound, which is a common pollutant in petrochemical wastewater. It has strong toxicity, has certain teratogenic, carcinogenic, and mutagenic effects on organisms, and has a Larger potential hazards can accumulate in higher organisms including humans through the food chain, and a large amount of quinoline-containing pollutants will pose a serious threat to human health and the natural environment. At present, quinoline is more and more widely used, and its consumption is increasing continuously. It persists in the environment and spreads continuously, and has become a common pollutant in the soil environment and water environment. It is difficult to achieve a good degr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/11C12N15/10
CPCC12N15/1003C12N15/11
Inventor 豆俊峰曹巍王营营朱宜丁爱中云影
Owner BEIJING NORMAL UNIVERSITY
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