High-speed Fourier stacking imaging device based on annular illumination and reconstruction method

Abstract

The invention discloses a high-speed Fourier stacking imaging device based on annular illumination and a reconstruction method. The device adopts an annular LED board as an illumination light source.The illumination numerical aperture of each LED on the ring is equal to the numerical aperture of an objective lens. Iterative reconstruction is realized through LED unit brightness calibration, LED location calibration and correction, original image acquisition, original image preprocessing and high resolution image initialization in order. According to the invention, the illumination angle of each LED on the ring is inscribed with the numerical aperture of the objective lens, so that a phase transfer function completely covers all low frequency parts of the phase spectrum; simply by taking asmall amount of low-resolution pictures of the bright field, the large-visual-field high-resolution phase distribution of an object can be stably and accurately reconstructed; and the device is suitable for label-free high-speed quantitative phase microscopy imaging of living cells.

Description

technical field [0001] The invention belongs to optical microscopic imaging technology, in particular to a ring-shaped lighting-based high-speed Fourier stack imaging device and a reconstruction method. Background technique [0002] Phase recovery is an important technology in optical measurement and imaging. No matter in the field of biomedicine or industrial detection, phase imaging technology is playing an important role. Throughout the development of optical measurement technology for nearly half a century, the most classic phase measurement method is interferometry. However, the disadvantages of interferometry are also very obvious: (1) Interferometry generally requires a highly coherent light source (such as a laser), which requires a more complex interferometric device; (2) The introduction of an additional reference optical path leads to the measurement environment The requirements become very stringent; (3) The coherent speckle noise introduced by the highly cohere...

AI Technical Summary

Problems solved by technology

However, the phase recovery accuracy of this method has not been verified, and there are often a large number of low-frequency phase reconstruction errors

In addition, because it needs to collect a large number of low-resolution images to reconstruct a high-resolution image, the imaging speed is slow, and it is difficult to meet the current needs of various high-speed quantitative phase microscopy imaging (such as label-free dynamic quantitative phase imaging of living cells)

Images