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Cell culture bottle for co-culturing attached cells and suspension cells

A technology of suspension cells and adherent cells, which is applied in the field of cell culture flasks, can solve problems such as co-cultivation of adherent cells and suspension cells, and achieve the goals of reducing the number of open operations, reducing the probability of cross-contamination, and improving the efficiency of interaction Effect

Pending Publication Date: 2018-04-20
湖北省银丰鼎诚生物工程有限公司 +1
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  • Description
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Problems solved by technology

[0009] The technical problem to be solved by the present invention is to provide a cell culture flask for co-cultivation of adherent cells and suspension cells. This device can allow the cytokines secreted by the two cells to osmotically interact through a semi-permeable membrane, and truly reflect the two types of cells. The interaction can well solve the problem of co-cultivation of adherent cells and suspension cells

Method used

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  • Cell culture bottle for co-culturing attached cells and suspension cells
  • Cell culture bottle for co-culturing attached cells and suspension cells

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Embodiment Construction

[0025] Below in conjunction with embodiment, further illustrate the present invention.

[0026] see figure 1 , figure 2 It can be seen that a cell culture bottle for the co-cultivation of adherent cells and suspension cells of the present invention consists of a bottle body 3, a bottle mouth 2 and a bottle cap 1; the bottle body 3 is provided with a bottle mouth 2, and the bottle body 3 is provided with a nano The semi-permeable membrane 5 and the semi-permeable nano-membrane 5 divide the space in the bottle body 3 into two parts, which are respectively used as a culture container 4 for adherent cells and a culture container 6 for suspended cells, and the bottle cap 1 is a gas-permeable bottle cap 1 .

[0027] In this technical scheme, a layer of nano-semipermeable membrane 5 is added in the middle on the basis of the traditional cell culture bottle, and the molecular permeation aperture of the nano-semi-permeable membrane 5 can be set according to needs. The size of the a...

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Abstract

The invention discloses a cell culture bottle for co-culturing attached cells and suspension cells. The cell culture bottle comprises a bottle body, wherein the bottle body is provided with a bottle opening and a bottle cap; the bottle body is internally provided with a nano semipermeable membrane; the nano semipermeable membrane is used for separating a space in the bottle body into two parts which are used as a culture container of the attached cells and a culture container of the suspension cells respectively; the bottle cap is an air permeable bottle cap. According to the cell culture bottle disclosed by the invention, the two types of cells do not influence each other in an operation process; however, cell factors secreted between the two types of cells can interact through the nano semipermeable membrane, and can really reflect mutual effects and influences between the two types of cells in a certain sense.

Description

technical field [0001] The invention relates to the field of biological experiment consumables, in particular to a cell culture bottle for co-culture of adherent cells and suspension cells. Background technique [0002] Cell culture flasks, as the name suggests, are used to culture cells. However, all cultures currently on the market can be classified into glass and plastic according to the material, and the bottom shape is square, rectangular, triangular, etc., and there are 5 to 500ml of bottles to choose from according to the volume of the bottle. In addition, after the surface is specially modified, the cells can be better adhered to the culture, so that according to the area of ​​the cells adhered to the wall, it can be divided into 25 ~ 175cm 2 Various. However, there is no cell flask that can co-culture two different culture types (adherent cells and suspension cells) at the same time. [0003] A semi-permeable membrane is a membrane that only allows certain molecu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M3/00C12M1/24
CPCC12M23/08C12M23/38C12M29/04C12M29/20C12M35/08C12M23/34
Inventor 曹启龙周芙玲魏永长宋现收曲廷瑜黄昕华徐峰波李海英
Owner 湖北省银丰鼎诚生物工程有限公司
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