Digital holographic microscopy device based on local hollow beam illumination and working method thereof

Abstract

The invention, which belongs to the field of digital holographic microscopy (DHM), relates to a digital holographic microscopy device based on local hollow beam illumination and a working method thereof. The digital holographic microscopy device is composed of a laser device, an optical beam splitter I, an optical beam splitter II, an optical reflector I, an optical reflector II, a dark field condenser, a microscope objective I, a microscope objective II, a spatial filter, a first lens L1, a second lens L2, a third lens L3, a computer, and other components. The digital holographic microscopy device is implemented as follows: after incidence of a generated local hollow light beam to the dark field condenser, an annular light cone illumination experiment sample is formed; with the digital holography, interference fringes of diffracted light and reference light of the sample are recorded to the computer by an optocoupler device; and then on the basis of a reconstructive technique, a three-dimensional image of an object is reproduced. Compared with the traditional digital holographic microscopy, the digital holographic microscopy device provided by the invention has the higher resolution and contrast ratio and is suitable for microcosmic fields such as observation of living cells and solvent particles in biomedical detection.

Description

technical field [0001] The present invention relates to a kind of digital holographic microscope technology recording the interference fringes of scattered light and spherical wave reference light obtained by illuminating experimental samples with localized hollow beams, in particular to a digital holographic microscope device and its working method based on localized hollow beams illumination , belonging to the field of digital holographic microscopy (DHM). The invention is applicable to research fields such as biology, biomedical detection, and environmental science. Background technique [0002] Digital holographic microscopy (DHM), which has been developed in recent years, has the advantages of low cost, simple operation, and can simultaneously obtain the quantitative intensity and position image information of the sample. This technology can be used for convenient filtering, phase distortion correction, automatic focusing, and expansion. Depth of focus and other operat...

AI Technical Summary

Problems solved by technology

[0003] The technical problem to be solved by the present invention is to provide a digital holographic microscope device based on local hollow beam illumination and its working method, in order to overcome the inner and outer radius of the ring-shaped illumination light existing in the existing dark-field digital holographic microscopy illumination light The invention can effectively improve the resolution of the system and enhance the contrast of the reproduced image due to problems such as poor controllability of the ring diameter ratio, uneven light waves, weak imaging contrast, and a large loss of light energy.

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