Method used for discovering and identifying liver cancer serum differential expression proteins and verifying marker proteins

Abstract

The invention discloses a method used for discovering and identifying liver cancer serum differential expression proteins and verifying marker proteins. According to the method, iTRAQ labeling MALDI-TOF MS / MS technology is adopted for discovering and identifying liver cancer serum differential expression proteins and MRM verifying of marker proteins. According to the method, searching on effectivetumor markers possesses important meaning on canceration mechanism study, disease early stage diagnosis, and prognosis. Selected novel liver cancer protein markers are subjected to in vivo and in vitro experiments such as gene knockin and knockout, and nude mice transplanted cancer, influences of blocking or up-regulating of expression of candidate genes on tumor biological behavior are observed,and key proteins capable of influencing human liver cancer occurrence and development are positioned, the effects of the key proteins in a system signal network is analyzed based on bioinformatics analysis, and the feasibility, the liver cancer diagnosis value, and the clinical significance of the key proteins in clinical applications including liver cancer prevention, early stage diagnosis, andprognosis is evaluated based on MRM absolute quantitative verification.

Description

technical field [0001] The invention relates to the fields of gene transcriptomics and proteomics, in particular to a method for discovering and identifying differentially expressed proteins in liver cancer serum and verifying marker proteins. Background technique [0002] In the 20th century, primary liver cancer has risen to become the second cancer killer in my country, second only to lung cancer in urban areas and gastric cancer in rural areas [1] . At present, about 35,000 people in Guangxi die of malignant tumors every year, among which, about 12,000 people die of liver cancer. It can be seen that liver cancer is the first cancer killer in Guangxi. In Guangxi, the hazards of liver cancer are very uneven. The counties and cities with high mortality rate of liver cancer are mainly concentrated in the southwestern part of Guangxi. The three counties of Fusui, Long'an and Wuming around Nanning have the highest incidence. The incidence density of liver cancer in this loca...

AI Technical Summary

Problems solved by technology

The high sensitivity of mass spectrometry can help people discover low-abundance proteins and peptides in biological samples in the stage of marker discovery. The dynamic range greatly affects the quantitative detection of low-abundance proteins and peptides in biological fluids by mass spectrometry

Although researchers have tried to use a variety of methods, including chromatographic separation as much as possible, removal of high-abundance proteins, etc., the precision and accuracy of the results are still far from meeting the requirements of analysis, until mass spectrometry multiple reaction monitoring (multiple reaction monitoring) The emergence of monitoring, MRM) technology

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