A kind of medicinal composition for treating heart disease

A pharmaceutical composition, a technology for heart disease, which can be used in drug combinations, pharmaceutical formulations, cardiovascular system diseases, etc., and can solve problems such as decreased cardiac function, inability to restore cardiac function, high blood sugar, and inability of damaged cells to regenerate by themselves.

Active Publication Date: 2021-05-18
NUMEN BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The manifestation of these pathological reactions will eventually lead to the decline of heart function, and after myocardial inflammation occurs, the damaged cells cannot reg...

Method used

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  • A kind of medicinal composition for treating heart disease
  • A kind of medicinal composition for treating heart disease
  • A kind of medicinal composition for treating heart disease

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0051] Embodiment 1: rat adipose stem cell extraction and experiment

[0052] Adipose stem cell extraction The fat in the abdominal cavity of 8-month-old Wistar strain male mice was removed by surgery, and the fat was cut into an appropriate size, and the fat was washed with physiological saline containing antibiotics, and the washed fat tissue was placed into Collagenase (0.01%) in physiological saline, heated and stirred in a 37°C water bath for about 1 hour, centrifuged at 3000rpm at room temperature for about 10 minutes, took out the lower sediment and placed it in a cell culture dish for cell culture.

[0053] 1. Adipose stem cell identification experiment

[0054] Adipose stem cells are cultured to the second generation before being reinfused into mice through the tail vein for autologous adipose stem cell transplantation. Before stem cell autotransplantation, the cultured adipose stem cells need to be identified to confirm that the transplanted cells are stem cells. Th...

Embodiment 2

[0071] Embodiment 2: animal experiment design and analysis

[0072] Two-month-old Wistar strain male mice (purchased from Green Four Seasons Company) were divided into four groups, namely the normal group, the diabetic group induced by STZ (55 mg / kg), the diabetic plus autologous adipose stem cell treatment group, and the diabetic plus green tea EGCG pretreatment group. Autologous fat stem cell therapy group, etc. The rats were raised in the animal room under a cycle of 12 hours during the day and 12 hours at night. During the feeding period, they had free access to food and water. Two rats were kept in one animal cage, and the animal cage was changed every two days during the feeding period. When the blood sugar of the rats in the diabetic group rose to 200mg / dl, they were deemed to have diabetes, and the rats in the diabetic group were treated with autologous stem cell transplantation one month later. The reinfusion of autologous stem cells was 1×10 per rat through the ...

Embodiment 3

[0080] Embodiment 3: animal cardiomyocyte cell culture and analysis

[0081] Embryonic rat myocardial transformed cell line H9c2cells (from ATCC CRL-1446) and adipose stem cells were cultured in a medium containing 10% fetal bovine serum (fetal bovine serum, FBS, Hyclone), 1% Antibiotic-Antimycotic (antibacterial-antifungal agent, Gibco) Dulbeco's Modified Eagle Medium (DMEM, Sigma), the incubator is set to 5% CO2, 37 °C constant temperature environment, and the accompanying culture solution is replaced 2 to 3 times a week. After culturing cardiomyocytes overnight with serum-free medium (serum-free medium), the cardiomyocytes were treated with drugs at different time points or at different drug concentrations.

[0082] 1. Survivin analysis of animal cardiomyocytes

[0083] After the animal experiment, the hearts of the rats in each group were dissociated and homogenized, and the expression levels of proteins related to survival in the heart tissue of the rats in each group we...

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Abstract

A pharmaceutical composition for repairing diabetic heart disease. The composition contains ester-type catechin (EGCG) and fat stem cells. The ester-type catechin (EGCG) can enhance the ability of fat stem cells and increase the ability of fat stem cells to repair damaged tissues .

Description

technical field [0001] The present invention provides a pharmaceutical composition for repairing diabetic heart disease and its application method, characterized in that the pharmaceutical composition for repairing diabetic heart disease contains fat stem cells pretreated with ester type catechin (EGCG), The ability to repair damaged tissue of the adipose stem cells pretreated with green tea catechins is significantly increased. Background technique [0002] Diabetes (Diabetes Mellitus, DM) is a disorder of sugar, protein and fat metabolism caused by the lack of insulin in the body or the inability of insulin to play a normal physiological role in the target cells. It has been clinically proven that many diseases will be accompanied by diabetes. However, these diseases are called complications of diabetes. Complications of diabetes include cardiovascular disease, kidney disease, peripheral vascular disease, eye disease, liver disease, or neuropathy or peripheral neuropathy....

Claims

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Application Information

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IPC IPC(8): C12N5/00A61K31/353A61P9/00
CPCA61K31/353C12N5/00
Inventor 刘绍毅黄志扬刘才睿陈冬生刘懿德
Owner NUMEN BIOTECH CO LTD
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