Preparation method and antioxidant application of bean taro leaf water extract

A technology of water extract and taro, applied in the field of medicine, can solve the problem that the antioxidant activity of taro leaf extract is rarely reported, and achieve the effects of protecting liver cells from oxidative damage, expanding application fields, and reducing the generation of reactive oxygen species.

Active Publication Date: 2021-01-26
ZHEJIANG UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Therefore, many nutrition and health food researchers are currently paying great attention to research and topics related to anti-oxidation and the occurrence and prevention of chronic diseases, but there are few reports on the antioxidant activity of bean taro leaf extract

Method used

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  • Preparation method and antioxidant application of bean taro leaf water extract
  • Preparation method and antioxidant application of bean taro leaf water extract
  • Preparation method and antioxidant application of bean taro leaf water extract

Examples

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Embodiment 1

[0052] Embodiment 1, a kind of preparation method of bean taro leaf water extract, carries out following steps successively:

[0053] 1) Mix taro leaves and water at a ratio of 1g:8ml, and use a juice extractor to beat; the obtained slurry is leached by ultrasonic (ultrasonic frequency of 40,000Hz) for 240min at 50°C, and the extract is extracted at 4000 rpm centrifuge for 30min,

[0054] The filter residue obtained by centrifugation replaces the taro leaves in the above steps and repeats the beating, ultrasonic extraction and centrifugation of the above steps; the number of repetitions is 3 times;

[0055] The supernatants obtained by the above 3 times of centrifugation were combined to carry out the following step 2).

[0056] 2), the obtained supernatant was concentrated by rotary evaporation in a rotary evaporator (the process parameters set by the rotary evaporation were temperature 38°C, vacuum degree -0.09MPa) until it was 10% of the original volume;

[0057] 3), the ...

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Abstract

The invention discloses a preparation method of a water extract of bean taro leaves, which comprises the following steps: 1), mixing bean taro leaves and water, beating, ultrasonic extraction, and centrifugation to obtain a supernatant; 2) making the supernatant 3), the concentrated solution is separated and purified by macroporous resin; 1% formic acid aqueous solution with volume concentration is used as eluent, and methanol aqueous solution containing 0.05% formic acid is used as eluent to collect the eluent; 4 ), concentrating the eluent by rotary evaporation to obtain a slurry-like concentrate; 5), pre-freezing the slurry-like concentrate, and then drying it into powder with a vacuum freeze dryer to obtain a water extract of bean taro leaves. The present invention also simultaneously provides the application of the above-mentioned bean taro leaf water extract in the preparation of an inhibitor of liver cell oxidative damage.

Description

technical field [0001] The invention relates to the field of medicine, in particular to a preparation method of a water extract of bean taro leaves and its anti-oxidation application. Background technique [0002] Apios americana Medikus is a perennial leguminous plant (Papilionaceae subfamily) native to the eastern part of North America from Canada to the southern part of Florida. Its edible part is the underground tuber, and its protein content is higher than that of other plant tubers. It is a crop with both nutrition and economic value, so it is often used as a staple food by Indians. Bean taro has been successfully introduced in China since 2009, and has been gradually promoted and planted in Fuyang, Jinhua, Longyou, Wenzhou and other places. Studies have shown that different parts of taro (flowers, vines, leaves, tubers) are rich in free amino acids, soluble proteins, polysaccharides, saponins, flavonoids, isoflavones, vitamin C, vitamin E and minerals. Among them, t...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K36/48A61P1/16A23L33/105A61K127/00
CPCA23V2002/00A61K36/48A61K2236/31A61K2236/51A61K2236/55A23L33/105A23V2200/32A23V2250/21
Inventor 郑晓冬楚强刘阳阳余露霜李永璐
Owner ZHEJIANG UNIV
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