Method for identifying authenticity of Indian pumpkin*Chinese pumpkin hybrid rootstock varieties

Abstract

The present invention relates to a method for identifying the authenticity of Cucurbita maxima*Cucurbita moschata hybrid rootstock varieties, and belongs to the field of biology. In the prior art, identification of the variety authenticity is increasingly important for the maintaining of the interests of producers and breeders; the Indian pumpkin*Chinese pumpkin hybrid rootstock varieties are more, and are similar in appearance; and the application of the morphological indexes to identify the authenticity has disadvantages of long time consuming and poor reliability. In order to solve the problems in the prior art, the invention provides the method for identifying the authenticity of the variety by using molecular markers. The technical scheme comprises that three rootstock varieties and control materials are subjected to PCT amplification with 5 pairs of SSR primers (SEQ ID NO.1-10), the generated 9 difference sites are assigned with 0 or 1, and a variety fingerprint for variety authenticity identification is constructed. According to the present invention, the Cucurbita maxima*Cucurbita moschata hybrid rootstock varieties such as Yongzhen 7, Yongzhen 8 and Yongzhen 10 are accurately and effectively identified and protected through the sensitive identification method at the low time cost and the low labor cost.

Description

1. Technical field [0001] The invention belongs to the field of biology, and in particular relates to a method for identifying the authenticity of rootstock varieties 'Yongzhen No. 7', 'Yongzhen No. 8' and 'Yongzhen No. 10' of Indian pumpkin x Chinese pumpkin hybrids of the Yongzhen series. 2. Background technology [0002] Continuous cropping obstacles caused by watermelon wilt are common in melon growing areas and are a major problem in melon production. According to statistics, the incidence rate of watermelon wilt can reach 10-30% every year, serious plots or even production failure, resulting in huge economic losses. At present, due to the limitation of land resources and the stubbornness of the pathogenic bacteria of Fusarium wilt, crop rotation and chemical control are not effective, and there are problems such as environmental pollution and food safety. Therefore, grafting is an effective means to overcome continuous cropping obstacles and Fusarium wilt in watermelon...

AI Technical Summary

Problems solved by technology

These methods are time-consuming, easily affected by the environment, and have poor reliability. Faced with a variety of India-China hybrid pumpkin rootstock materials with similar appearance and phenotype, it is difficult to identify the authenticity.

[0005] The new rootstock varieties 'Yongzhen No. 7', 'Yongzhen No. 8' and 'Yongzhen No. 10' have only been identified by field morphological purity so far, and there is still a lack of convenient and effective methods for authenticity identification of varieties, which is far from enough for variety protection. not enough

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