Antigen protein of avibacterium paragallinarum and application of antigen protein

Abstract

The invention discloses an antigen protein of avibacterium paragallinarum and application of the antigen protein. Amino acid sequences of the antigen protein are shown as SEQ ID NO.1. The invention further provides a reagent kit for detecting antibodies of the avibacterium paragallinarum and a vaccine preparation for the avibacterium paragallinarum. The reagent kit can be used for detecting the antibodies induced by outer membrane proteins of A-type thalli, B-type thalli and C-type thalli of the avibacterium paragallinarum or assisting in detecting the antibodies induced by the outer membraneproteins of the A-type thalli, the B-type thalli and the C-type thalli of the avibacterium paragallinarum. The antigen protein, the application, the reagent kit and the vaccine preparation have the advantages that immune states of chicken can be judged by the aid of detection results, so that corresponding measures can be adopted by farmers in the poultry industry; the reagent kit contains negative control serum and positive control serum, and accordingly the accuracy and the reliability of the detection results can be guaranteed; the vaccine preparation can be used for being vaccinated in thechicken, and accordingly the chicken can be effectively protected against infection of the A-type, B-type and C-type avibacterium paragallinarum.

Description

technical field [0001] The invention relates to the technical field of biological detection, in particular to an antigenic protein of Avian bacillus paragallinarum and its application. Background technique [0002] Avibacterium paragallinarum (Apg) is a small Gram-negative bacterium with a length of about 1-3 μm and a width of 0.4-0.8 μm. It has no flagella and does not form spores. Virulent strains often have capsules. The culture conditions of Aviola paragallinarum are relatively harsh, and NAD needs to be added to the medium, but NAD-independent isolates were found in South Africa, indicating that NAD is not an essential nutrient. The bacteria are facultatively anaerobic and require an anaerobic environment or 3%-10% CO when cultured on a solid medium 2 . [0003] Avibacterium paragallinarum is the pathogenic bacterium of infectious coryza (IC) in chickens, which causes a respiratory infectious disease in chickens. It causes chickens to sneeze and discharge thin watery...

AI Technical Summary

Problems solved by technology

This method has the deficiencies in the following aspects: 1. Fresh chicken red blood cells must be used when evaluating the vaccine effect of Apg A type bacteria, so it is necessary to raise test chickens all year round, and to ensure the health of chickens for blood supply; blood collection and blood processing, etc. The process is time-consuming and labor-intensive; 2. Differences between batches of chicken red blood cells affect the test results; 3. This method ultimately relies on the subjective judgment of the antibody titer by the tester, and it is difficult to obtain a stable standard

However, this method needs to ensure that the type-specific monoclonal antibody and the antibody in the serum sample can all bind to the same epitope. When the epitope is lost due to the variation of the bacteria, it is impossible to detect the infection caused by the bacteria or vaccination. Antibodies produced, this method is prone to false negative results

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