Microelectrode fluidic chip and single-cell electroporation device with adjustable parameters

Abstract

The invention discloses a microelectrode fluidic chip and a parameter-adjustable single cell electroporation device. The microelectrode fluidic chip comprises a transparent substrate and a microfluidchannel layer, wherein the microfluid channel layer is positioned above the transparent substrate; the transparent substrate is integrated with microelectrodes; the microelectrodes comprise interdigital electrodes and a parallel electrode; the interdigital electrodes and the parallel electrode are distributed under a microfluidic electroporation channel, and are sequentially distributed from a cell suspension inlet to a cell outlet. The parameter-adjustable single cell electroporation device comprises a regulated power supply module, a function signal generation module, a printed circuit board(PCB) control circuit and digital display module, a chip carrier and the microelectrode fluidic chip. The parameter-adjustable single cell electroporation device can be used for sequentially carryingout electroporation treatment on single cells, thus reducing cell damage and even mortality and increasing the cell electrical transfection efficiency; by controlling the types and numbers of the electrodes which are selected to be connected with a circuit, the single cell electroporation device changes the frequency, amplitude and duty ratio of electrical signals, thus optimizing the electroporation parameters for different cell lines.

Description

technical field [0001] The invention belongs to the fields of microfluidic technology, single-cell electroporation and electrotransfection, and in particular relates to a parameter-adjustable single-cell electroporation device based on a microelectrode fluidic chip. Background technique [0002] Microfluidics is the science and technology involved in processing or manipulating systems with volumes ranging from nanoliters to microliters of fluids using microfluidic channels ranging in size from a few microns to hundreds of microns. Emerging interdisciplinary subjects in the fields of science, microelectronics, biology, chemistry, new materials, etc. Chips based on microfluidic technology are characterized by miniaturization and integration, and are often called lab on a chip (Lab on a Chip) and micro total analysis system (microTAS). Microfluidic technology is considered to have great development potential and broad application prospects in biomedical research. [0003] Whe...

AI Technical Summary

Problems solved by technology

[0005] In traditional cell electroporation instruments, the electrode size is large, and the electrode spacing is still at the macro scale, at the scale of ten millimeters, while the cell size is at the micron scale. Therefore, the applied voltage is large, about several hundred volts, and the electric field is not uniform. The electric field environment of the cells is different, causing the cells near the electrode to die easily, and the cells located in the weaker electric field cannot be perforated and transfected, and the survival rate and transfection efficiency are relatively low

The parameters of the existing microfluidic electroporation device, such as the electrode spacing, are fixed, and the operability of the experiment and the adjustability of the electroporation parameters are limited by the design of the chip, which lacks versatility

In addition, key parameters such as the frequency and amplitude of electrical signals of commercialized electrotransfection instruments are set by the manufacturer, and customers cannot optimize and adjust them. For special cells, such as primary cells and immune cells, the transfection efficiency is not high

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