Method for preparing cigarette radix saposhnikoviae extract by virtue of immobilized acetobacter

A technology of root extract and acetic acid bacteria, which is applied in the field of preparation of root extracts for tobacco, can solve the problems of rare reports of natural flavors for tobacco, and achieve the goal of improving throat comfort, high efficiency, and increased applicability Effect

Inactive Publication Date: 2018-02-09
WUHAN HUANGHELOU FLAVOR & SPICES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Acetobacter is a general term for a type of bacteria that can use fermentable carbohydrates to produce a large amount of acetic acid and other organic acids. Acetobacter is widely used in vinegar, fruit vinegar and other brewing industries, but it is rarely reported in the preparation of natural flavors for tobacco

Method used

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  • Method for preparing cigarette radix saposhnikoviae extract by virtue of immobilized acetobacter

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Embodiment 1, a kind of method that immobilized acetic acid bacterium prepares Radix Radix Radix Sinensis for tobacco use comprises the following steps:

[0022] (1) Add 5 times of water by weight to the chopped Radix Radix Radix Radix Radix Radix Radix and Radix, reflux extraction at 100 ℃ for 2 times, each extraction time is 3 hours, cool to room temperature after extraction, and filter to obtain the extract, combine Extract twice to obtain Radix Radix Radix Radix Radix Radix A., and the conditions of the two extractions are exactly the same;

[0023] (2) Mix Radix Radix Radix Radix Radix A. and the PVA-SA immobilized acetic acid bacteria pellets prepared by the above method according to the mass ratio of 5:1, ferment for 4 days at a temperature of 30°C and a stirring speed of 5 rpm, and then place Store in a 4°C freezer for 7 days and then place at room temperature for 1 to 2 days;

[0024] (3) Filter the fermented liquid after step (2) fermentation to obtain a clar...

Embodiment 2

[0026] Embodiment 2, a kind of method that immobilized acetic acid bacterium prepares Radix Radix Radix Sinensis for tobacco use comprises the following steps:

[0027] (1) Add water 4 times its weight to the chopped Radix Radix Radix Radix Radix Radix Radix Radix and Radix, reflux extraction at 98 ℃ for 3 times, each extraction time is 2 hours, cool to room temperature after extraction, and filter to obtain the extract, combine Three times of extracting solution, obtain Radix Radix Radix Radix Radix Radix A.; The conditions of three extractions are exactly the same;

[0028] (2) Mix Radix Radix Radix Radix Radix A. with the PVA-SA immobilized acetic acid bacteria pellets prepared in the above method according to the mass ratio of 8:1, and ferment for 3 days at a temperature of 35°C and a stirring speed of 2rpm. , and then stored in a 4°C freezer for 10 days, and then at room temperature for 1 to 2 days.

[0029](3) Filter the fermented liquid after step (3) fermentation to o...

Embodiment 3

[0031] Embodiment 3, a kind of method that immobilized acetic acid bacteria prepares Radix Radix Radix Sativa for tobacco use comprises the following steps:

[0032] (1) Add 3 times the weight of water to the chopped Radix Radix Radix, reflux and extract 3 times at 95°C, each extraction time is 4 hours, cool to room temperature after extraction, and filter to obtain the extract, combine three times extracting liquid to obtain Radix Radix Radix Radix Radix Radix Radix Radix Radix Radix A.; three extraction conditions are exactly the same;

[0033] (2) Mix Radix Radix Radix Radix Radix A. with the PVA-SA immobilized acetic acid bacteria pellets prepared by the above method at a mass ratio of 10:1, ferment for 2 days at a temperature of 25°C and a stirring speed of 3rpm, and then place Store in a 4°C freezer for 14 days, and then place it at room temperature for 1 to 2 days;

[0034] (3) Filter the fermented liquid after step (2) fermentation to obtain clarified fermented liquid...

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Abstract

The invention provides a method for preparing a cigarette radix saposhnikoviae extract by virtue of immobilized acetobacter. Specifically, the preparation method comprises the following steps: (1) conducting water extraction on prepared radix saposhnikoviae for several times at 95-100 DEG C, and combining extracting solutions, so that a radix saposhnikoviae extract A is obtained; (2) inoculating abroth medium with acetobacter, conducting culture and centrifuging, so that a wet bacterium is obtained; adding the wet bacterium to a mixed solution of polyvinyl alcohol and sodium alginate, and conducting cross-linking in a boric acid saturated solution containing 1-3% of CaCl2 and a Na2SO4 solution, so that PVA-SA immobilized acetobacter spheres are obtained; and (3) mixing the radix saposhnikoviae extract A and the immobilized acetobacter spheres at the mass ratio of (5-10) to 1 and conducting fermentation, then conducting filtering so as to obtain clear fermentation liquid, adding an alcohol solution and promoting precipitation, conducting filtering and concentrating supernatant liquid, so that the cigarette radix saposhnikoviae extract is obtained. The cigarette radix saposhnikoviaeextract provided by the invention, when added to cigarettes, can enrich cigarette fragrance, improve smell of medicinal herbs and sour aroma, offer soft and delicate smoke, improve throat comfort andmask offensive odor.

Description

technical field [0001] The invention relates to the field of tobacco additives, in particular to a preparation method for preparing tobacco root extracts by using immobilized acetic acid bacteria. Background technique [0002] Flavoring is a key link in the cigarette process, and it is one of the effective means to improve the determinant of product style and improve the quality of cigarette aroma. At present, flavors and fragrances for tobacco are mainly prepared through extraction and chemical synthesis of natural animals and plants. Due to limited natural plant resources and harsh conditions for chemical synthesis. With the development of biotechnology, in recent years, the use of microbial fermentation to prepare tobacco flavor raw materials provides an economical and efficient way for the production of tobacco flavor raw materials, and develops some new types of tobacco flavor raw materials that cannot be produced by traditional methods. [0003] Microbial immobilizat...

Claims

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Application Information

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IPC IPC(8): C12P1/04C12N11/10C12N11/08A24B3/12A24B15/24C12R1/02
CPCA24B3/12A24B15/24C12N11/08C12N11/10C12P1/04C12N1/205C12R2001/02
Inventor 黄龙庞登红王珊张磊潘妮
Owner WUHAN HUANGHELOU FLAVOR & SPICES
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