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Self-injection immunoassay paper chip and preparation method thereof

An immunodetection, paper chip technology, applied in analytical materials, instruments, etc., to achieve the effects of rapid trace detection, rapid and efficient detection, and easy operation

Active Publication Date: 2020-12-22
INST OF ELECTRONICS CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the above methods all require pretreatment of the detected antigen or antibody, so they have certain limitations in real-time detection.

Method used

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  • Self-injection immunoassay paper chip and preparation method thereof
  • Self-injection immunoassay paper chip and preparation method thereof
  • Self-injection immunoassay paper chip and preparation method thereof

Examples

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preparation example Construction

[0043] In order to achieve the above object, the present invention also proposes a method for preparing a self-introducing immunoassay paper chip, which is used to prepare the above-mentioned self-introducing immunoassay paper chip, comprising the following steps: the pre-set microfluidic channel After printing on the surface of the microfluidic layer by a wax spray printer, heat the microfluidic layer to make the area where the microfluidic channel of the microfluidic layer is hydrophobic; Print the working electrode, the counter electrode and the reference electrode; modify the electroactive compound on the surface of the working electrode; integrate and package the microfluidic layer, auxiliary electrode layer, detection layer and working electrode layer; the self-sampling immunoassay paper chip is used At this time, the antibody is dropped onto the surface of the working electrode modified with the electroactive compound substance.

[0044] Wherein, the above-mentioned ele...

Embodiment 1

[0067] This example proposes an automatic trace-amount rapid electrochemical immunological detection paper chip and its preparation method, which is used for the trace-amount rapid electrochemical detection of CEA and NSE two parameters at the same time automatic sampling. Specifically, such as figure 1 structure shown.

[0068] The preparation and use process of the automatic type trace fast electrochemical immunological whole blood detection paper chip for detecting tumor markers is as follows:

[0069] (1) Carcinoembryonic antigen (CEA) and neuron-specific ene purifying enzyme (NSE) were selected for joint determination;

[0070] (2) Preparation of paper chips for automatic trace rapid electrochemical immunological detection of whole blood: on the surface of the corresponding filter paper, the designed channel pattern is printed on the surface of the paper with a wax spray printer; it is put into the oven, due to Heating will melt the wax on the surface of the paper and pe...

Embodiment 2

[0076] This embodiment proposes an automatic type trace fast electrochemical immunological whole blood detection paper chip and its preparation method. The automatic type trace fast electrochemical immunological whole blood detection paper chip is used to detect neurotrophic factor (BDNF). Its preparation and use process are:

[0077] (1) Select BDNF parameters to measure;

[0078] (2) Preparation of paper chips for automatic trace rapid electrochemical immunological detection of whole blood: on the surface of the corresponding filter paper, the designed channel pattern is printed on the surface of the paper with a wax spray printer; it is put into the oven, due to Heating will melt the wax on the surface of the paper and penetrate into the inside of the paper, so that the wax-sprayed area becomes a hydrophobic area, and the non-wax-sprayed area becomes a hydrophilic area. Therefore, by accurately controlling the temperature and time of heating, the hydrophilicity can be preci...

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Abstract

The invention discloses an automatic sample introduction immunodetection paper chip and a production method thereof. The automatic sample introduction immunodetection paper chip is used for detectingthe antigen concentration, and comprises a micro-fluidic layer, an auxiliary electrode layer, a detection layer and a working electrode layer from top to bottom along a lamination direction; the micro-fluidic layer is characterized in that the micro-fluidic layer has a micro-fluidic channel and a sample introduction hole which are connected through a communicating channel, the micro-fluidic channel is used for automatically sampling, and the sample introduction hole is used for introducing sampled tissues to be detected into a detection area; and the working electrode layer is characterized inthat the surface of the working electrode layer is modified with an electroactive composite substance, the surface of the electroactive composite substance is used for immobilizing an antibody; and the auxiliary electrode layer, the detection layer and the working electrode layer form the detection area, and the concentration of the antigen in a liquid to be detected is detected based on the antigen-antibody specific binding principle and the characteristics of the electroactive composite substance through an electrochemical technology by using an electrochemical detection circuit. The respective advantages of a micro-fluidic technology and paper are combined, and automatic sample introduction is realized through the arranged micro-fluidic channel and the sample introduction hole, so thechip is easy to operate, and does not need professional operators.

Description

technical field [0001] The invention belongs to the field of biological detection, and more specifically relates to a self-introducing immune detection paper chip and a preparation method thereof. Background technique [0002] Antigen refers to a substance that can induce an immune response. Most antigens are specific and can specifically bind to antibodies. There are many types of antigens, including proteins and polypeptides. The content of certain antigens, such as disease markers, is of great significance to human health. The detection of these substances can be used for mechanism research, early diagnosis, early intervention and treatment of some major diseases. [0003] Currently, there are a variety of immunoassay methods available for high-sensitivity detection of antigens, including radioimmunoassay, enzyme-linked immunoassay (ELISA), electrochemiluminescence immunoassay, etc. However, the above methods all require pretreatment of the detected antigen or antibody...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N35/00
CPCG01N35/00029G01N2035/00108
Inventor 王杨蔡新霞罗金平刘军涛徐辉任孔壮樊艳
Owner INST OF ELECTRONICS CHINESE ACAD OF SCI
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