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Phylogenetic tree and identification method for identifying dendrobium huoshanense or dendrobium candidum

A phylogenetic tree, the technology of Dendrobium huoshanensis, which is applied in the directions of biochemical equipment and methods, microbial determination/inspection, DNA/RNA fragments, etc., can solve problems such as difficulty in identifying the appearance of Dendrobium huoshanense and Dendrobium candidum, and achieve rapid identification, consumption and consumption. Short time, high sensitivity effect

Inactive Publication Date: 2018-01-26
WEST ANHUI UNIV
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Problems solved by technology

Because it is difficult to identify Dendrobium Huoshanense and Dendrobium candidum through appearance, the traditional identification method has certain limitations.

Method used

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  • Phylogenetic tree and identification method for identifying dendrobium huoshanense or dendrobium candidum
  • Phylogenetic tree and identification method for identifying dendrobium huoshanense or dendrobium candidum

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Embodiment

[0023] 1. Construction method of phylogenetic tree for identification of Dendrobium huoshanense

[0024] Specific DNA sequences of Dendrobium Huoshanense and some related species were found from GenBank or sequenced in this study, as shown in Table 1 below. Among them, the haplotype sequences sequenced in this study, including Hap5, Hap6 and Hap7, correspond to sequence 1, sequence 2 and sequence 3 in the sequence listing, respectively.

[0025] Table 1 Specific DNA sequences of Dendrobium Huoshanense, Dendrobium officinale and some similar species

[0026]

[0027] Phylogenetic analysis was carried out by Bayesian inference (BI) and maximum parsimony (MP) respectively, and two phylogenetic trees of BI and MP were constructed. When constructing the phylogenetic tree, two specific DNA sequences of Dendrobium leptocladum were used as outgroups, and the GenBank accession numbers were AF521612 and EU840697 respectively. The BI tree was constructed with MrBayes version 3.1.2 so...

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Abstract

The invention discloses a phylogenetic tree and an identification method for identifying dendrobium huoshanense or dendrobium candidum, and belongs to the technical field of identification of the dendrobium huoshanense or the dendrobium candidum. The phylogenetic tree uses a specific DNA sequence of each of the dendrobium huoshanense, the dendrobium candidum and some similar species as an ingroup,and two specific DNA sequences of dendrobium leptocladum as an outgroup. The specific DNA sequence of the dendrobium huoshanense is obtained by sequencing, and the specific DNA sequences of some similar species of the dendrobium huoshanense and the dendrobium leptocladum are from GenBank. Phylogenetic analysis is performed according to a Bayesian method and a simple method to establish a BI phylogenetic tree and an MP phylogenetic tree respectively. Through establishment of the phylogenetic tree of the dendrobium huoshanense, the dendrobium candidum and some similar species and through alignment sorting and comparison of the specific DNA sequences of the dendrobium huoshanense and the dendrobium candidum, the dendrobium huoshanense and the dendrobium candidum can be rapidly and accuratelyidentified and a novel method is provided for identifying the dendrobium huoshanense.

Description

technical field [0001] The invention relates to the technical field of identification of Dendrobium Huoshanense or Dendrobium candidum, in particular to a phylogenetic tree and an identification method for identifying Dendrobium Huoshanense or Dendrobium candidum. Background technique [0002] Dendrobium huoshanense (Dendrobium huoshanense) is a unique plant in the Dabie Mountains of Anhui Province, and is a precious Chinese herbal medicine under national key protection, belonging to the genus Dendrobium (Orchidaceae). Dendrobium catenatum is a closely related species of Dendrobium huoshanense. The morphological characteristics of the two are relatively similar. After making a maple bucket, it is difficult to distinguish the two through their appearance. As a result, Dendrobium candidum has become one of the confused products of D. huoshanense in the market. Because it is difficult to identify Dendrobium Huoshanense and Dendrobium candidum by appearance, the traditional iden...

Claims

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Application Information

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IPC IPC(8): C12Q1/6895C12N15/11
Inventor 赵群孙传伯
Owner WEST ANHUI UNIV
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